Institute of Microbiology and Virology, Riga Stradins University, 5 Ratsupites Street, 1067 Riga, Latvia.
Latvian Biomedical Research and Study Centre, 1 Ratsupites Street k-1, 1067 Riga, Latvia.
Int J Mol Sci. 2022 Mar 22;23(7):3434. doi: 10.3390/ijms23073434.
Chemokines and their receptors regulate the migration of immune cells and the dissemination of cancer cells. CCR1, CCR2, CCR3, and CCR5 all belong to a single protein homology cluster and respond to the same inflammatory chemokines. We previously reported that CCR1 and CCR2B are induced upon Epstein-Barr virus (EBV) infection of B cells in vitro. EBV is present in almost all cases of endemic Burkitt lymphoma (BL); however, the contribution of EBV in the pathogenesis of the disease is not fully understood. Here, we analyzed the relation of the expression of , , , and , the EBV DNA load and expression of EBV latent genes in nine EBV-carrying and four EBV-negative BL cell lines. We revealed that is expressed at high mRNA and protein levels in two CD10-negative BL cell lines with co-expression of the EBV latent genes EBNA2, LMP1, and LMP2. Low levels of transcripts were found in three BL cell lines. and transcripts were hardly detectable. Our data suggest that in vivo, CCR1 may be involved in the dissemination of BL cells and in the selection of BL cells with restricted EBV gene expression programs.
趋化因子及其受体调节免疫细胞的迁移和癌细胞的扩散。CCR1、CCR2、CCR3 和 CCR5 均属于单一蛋白同源簇，对相同的炎症趋化因子作出反应。我们之前曾报道，CCR1 和 CCR2B 在体外 B 细胞感染 EBV 时被诱导。几乎所有地方性 Burkitt 淋巴瘤（BL）病例中均存在 EBV；然而，EBV 在疾病发病机制中的作用尚未完全阐明。在此，我们分析了 9 株 EBV 携带和 4 株 EBV 阴性 BL 细胞系中 、 、 、和 EBV DNA 负荷及 EBV 潜伏基因表达的关系。我们揭示，在共表达 EBV 潜伏基因 EBNA2、LMP1 和 LMP2 的 2 株 CD10 阴性 BL 细胞系中， 呈高 mRNA 和蛋白水平表达。在 3 株 BL 细胞系中发现 转录物水平较低。 和 转录物几乎检测不到。我们的数据表明，在体内，CCR1 可能参与 BL 细胞的播散，并参与 BL 细胞中具有受限 EBV 基因表达程序的选择。
