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MYB2 通过直接激活. 中的蛋白酶表达对绒毡层 PCD 和花粉发育很重要。

MYB2 Is Important for Tapetal PCD and Pollen Development by Directly Activating Protease Expression in .

机构信息

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China.

National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.

出版信息

Int J Mol Sci. 2022 Mar 24;23(7):3563. doi: 10.3390/ijms23073563.

DOI:10.3390/ijms23073563
PMID:35408924
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8998314/
Abstract

Tapetal programmed cell death (PCD) is a complex biological process that plays an important role in pollen formation and reproduction. Here, we identified the MYB2 transcription factor expressed in the tapetum from stage 5 to stage 11 that was essential for tapetal PCD and pollen development in Arabidopsis thaliana. Downregulation of MYB2 retarded tapetal degeneration, produced defective pollen, and decreased pollen vitality. EMSA and transcriptional activation analysis revealed that MYB2 acted as an upstream activator and directly regulated expression of the proteases CEP1 and βVPE. The expression of these proteases was lower in the buds of the myb2 mutant. Overexpression of either/both CEP1 or/and βVPE proteases partially recover pollen vitality in the myb2 background. Taken together, our results revealed that MYB2 regulates tapetal PCD and pollen development by directly activating expression of the proteases CEP1 and βVPE. Thus, a transcription factor/proteases regulatory and activated cascade was established for tapetal PCD during another development in Arabidopsis thaliana. Highlight: MYB2 is involved in tapetal PCD and pollen development by directly regulating expression of the protease CEP1 and βVPE and establishes a transcription factor/proteases regulatory and activated cascade.

摘要

绒毡层程序性细胞死亡（PCD）是一个复杂的生物学过程，在花粉形成和生殖中起着重要作用。在这里，我们鉴定了 MYB2 转录因子在拟南芥绒毡层中的表达，从第 5 期到第 11 期，该因子对绒毡层 PCD 和花粉发育是必需的。MYB2 的下调延迟了绒毡层的退化，产生了有缺陷的花粉，并降低了花粉活力。EMSA 和转录激活分析表明，MYB2 作为一个上游激活子，直接调控蛋白酶 CEP1 和 βVPE 的表达。在 myb2 突变体的芽中，这些蛋白酶的表达较低。CEP1 和/或βVPE 蛋白酶的过表达部分恢复了 myb2 背景下的花粉活力。总之，我们的结果表明，MYB2 通过直接激活蛋白酶 CEP1 和 βVPE 的表达来调节绒毡层 PCD 和花粉发育。因此，在拟南芥的另一个发育过程中，建立了一个转录因子/蛋白酶的调控和激活级联反应。

重点

MYB2 通过直接调控蛋白酶 CEP1 和 βVPE 的表达参与绒毡层 PCD 和花粉发育，并建立了一个转录因子/蛋白酶的调控和激活级联反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/41d6c083138a/ijms-23-03563-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/5493f90bd670/ijms-23-03563-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/919d6cbdcf85/ijms-23-03563-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/c76e31608608/ijms-23-03563-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/e1c196ba92d0/ijms-23-03563-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/1421a9c969bf/ijms-23-03563-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/fa4389982f6e/ijms-23-03563-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/e493366d4b7a/ijms-23-03563-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/41d6c083138a/ijms-23-03563-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/5493f90bd670/ijms-23-03563-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/919d6cbdcf85/ijms-23-03563-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/c76e31608608/ijms-23-03563-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/e1c196ba92d0/ijms-23-03563-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/1421a9c969bf/ijms-23-03563-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/fa4389982f6e/ijms-23-03563-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/e493366d4b7a/ijms-23-03563-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0c1/8998314/41d6c083138a/ijms-23-03563-g008.jpg

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