College of Life Sciences, Nankai University, Tianjin 300071, China.
Int J Mol Sci. 2024 Apr 8;25(7):4111. doi: 10.3390/ijms25074111.
is a prized traditional Chinese medicinal plant species. Its red storage roots are primarily used for the treatment of cardiovascular and cerebrovascular diseases. In this study, a transcription factor gene was cloned and introduced into for ectopic expression. Overexpression of enhanced salt stress resistance in , leading to a more resilient phenotype in transgenic plants exposed to high-salinity conditions. Physiological experiments have revealed that overexpression of can decrease the accumulation of reactive oxygen species (ROS) during salt stress, boost the activity of antioxidant enzymes, and mitigate oxidative damage to cell membranes. In addition, overexpression of promotes the synthesis of tanshinones and phenolic acids by upregulating the expression of biosynthetic pathway genes, resulting in increased levels of these secondary metabolites. In summary, our findings demonstrate that not only enhances plant tolerance to salt stress, but also increases the accumulation of secondary metabolites in . Our study lays a solid foundation for uncovering the molecular mechanisms governed by and holds significant implications for the molecular breeding of high-quality varieties.
丹参是一种珍贵的传统中药植物。其红色的根状茎主要用于治疗心脑血管疾病。在本研究中，克隆并导入了一个转录因子基因 到 中进行异位表达。在高盐胁迫条件下，过表达 增强了 对盐胁迫的抗性，导致转基因植株表现出更强的抗逆表型。生理实验表明，过表达 可以减少盐胁迫过程中活性氧（ROS）的积累，提高抗氧化酶的活性，减轻细胞膜的氧化损伤。此外，过表达 通过上调生物合成途径基因的表达促进丹参酮和酚酸的合成，从而增加这些次生代谢物的含量。综上所述，我们的研究结果表明， 不仅增强了植物对盐胁迫的耐受性，而且增加了 中次生代谢物的积累。我们的研究为揭示 调控的分子机制奠定了基础，对高品质 品种的分子育种具有重要意义。
