Integrated OMICs Approach for the Group 1 Protease Mite-Allergen of House Dust Mite .

House dust mites (HDMs) are one of the most important allergy-causing agents of asthma. In central Taiwan, the prevalence of sensitization to (), a particular mite species of HDMs, is approximately 80% and is related to the IgE crossing reactivity of () and (). Integrated OMICs examination was used to identify and characterize the specific group 1 mite-allergic component (). De novo draft genomic assembly and comparative genome analysis predicted that the full-length allergen gene is 321 amino acids in silico. Proteomics verified this result, and its recombinant protein production implicated the cysteine protease and α chain of fibrinogen proteolytic activity. In the sensitized mice, pathophysiological features and increased neutrophils accumulation were evident in the lung tissues and BALF with the combination of and 2 inhalation, respectively. Principal component analysis (PCA) of mice cytokines revealed that the cytokine profiles of the allergen-sensitized mice model with combined and 2 were similar to those with alone but differed from those with alone. Regarding the possible sensitizing roles of in the cells, the fibrinogen cleavage products (FCPs) derived from combined and induced the expression of pro-inflammatory cytokines IL-6 and IL-8 in human bronchial epithelium cells. biologically functions as a cysteine protease and contributes to the α chain of fibrinogen digestion in vitro. The combination of and 2 could induce similar cytokines expression patterns to in mice, and the FCPs derived from has a synergistic effect with to induce the expression of pro-inflammatory cytokines in human bronchial epithelium cells.