• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

扰乱/抑制淀粉代谢并赋予水稻增强的抗爆裂能力。

Disruption of / Represses Starch Metabolism and Confers Enhanced Blast Resistance in Rice.

机构信息

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China.

出版信息

Int J Mol Sci. 2022 Mar 30;23(7):3827. doi: 10.3390/ijms23073827.

DOI:10.3390/ijms23073827
PMID:35409186
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8998287/
Abstract

Catalases (CATs) are important self-originating enzymes and are involved in many of the biological functions of plants. Multiple forms of CATs suggest their versatile role in lesion mimic mutants (LMMs), H2O2 homeostasis and abiotic and biotic stress tolerance. In the current study, we identified a large lesion mimic mutant9428 (llm9428) from Ethyl-methane-sulfonate (EMS) mutagenized population. The llm9428 showed a typical phenotype of LMMs including decreased agronomic yield traits. The histochemical assays showed decreased cell viability and increased reactive oxygen species (ROS) in the leaves of llm9428 compared to its wild type (WT). The llm9428 showed enhanced blast disease resistance and increased relative expression of pathogenesis-related (PR) genes. Studies of the sub-cellular structure of the leaf and quantification of starch contents revealed a significant decrease in starch granule formation in llm9428. Genetic analysis revealed a single nucleotide change (C > T) that altered an amino acid (Ala > Val) in the candidate gene (Os03g0131200) encoding a CATALASE C in llm9428. CRISPR-Cas9 targetted knockout lines of LLM9428/OsCATC showed the phenotype of LMMs and reduced starch metabolism. Taken together, the current study results revealed a novel role of OsCATC in starch metabolism in addition to validating previously studied functions of CATs.

摘要

过氧化氢酶（CATs）是重要的内源性酶，参与植物的许多生物学功能。多种形式的 CATs 表明它们在病变模拟突变体（LMMs）、H2O2 动态平衡以及非生物和生物胁迫耐受中的多功能作用。在本研究中，我们从乙磺酸（EMS）诱变群体中鉴定出一个大的病变模拟突变体 9428（llm9428）。llm9428 表现出典型的 LMMs 表型，包括农艺产量性状下降。组织化学分析显示，与野生型（WT）相比，llm9428 叶片中的细胞活力降低，活性氧（ROS）增加。llm9428 表现出增强的稻瘟病抗性和增加的病程相关（PR）基因的相对表达。对叶片亚细胞结构的研究和淀粉含量的定量表明，llm9428 中淀粉颗粒形成明显减少。遗传分析显示候选基因（编码 CATALASE C 的 Os03g0131200）中的一个单核苷酸变化（C > T）改变了一个氨基酸（Ala > Val）。CRISPR-Cas9 靶向敲除 llm9428/OsCATC 的突变体显示出 LMMs 的表型和减少的淀粉代谢。总之，本研究结果揭示了 OsCATC 在淀粉代谢中的新作用，除了验证 CATs 的先前研究功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/0c9551b2cbd3/ijms-23-03827-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/e36a9f217d94/ijms-23-03827-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/84168e8f9f5e/ijms-23-03827-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/cef2cea45f2a/ijms-23-03827-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/a31ffac4837b/ijms-23-03827-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/616fb0db3985/ijms-23-03827-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/a0ccc008e3d2/ijms-23-03827-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/0c9551b2cbd3/ijms-23-03827-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/e36a9f217d94/ijms-23-03827-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/84168e8f9f5e/ijms-23-03827-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/cef2cea45f2a/ijms-23-03827-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/a31ffac4837b/ijms-23-03827-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/616fb0db3985/ijms-23-03827-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/a0ccc008e3d2/ijms-23-03827-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4176/8998287/0c9551b2cbd3/ijms-23-03827-g007.jpg

相似文献

1
Disruption of / Represses Starch Metabolism and Confers Enhanced Blast Resistance in Rice.扰乱/抑制淀粉代谢并赋予水稻增强的抗爆裂能力。
Int J Mol Sci. 2022 Mar 30;23(7):3827. doi: 10.3390/ijms23073827.
2
Identification of a Novel Semi-Dominant Spotted-Leaf Mutant with Enhanced Resistance to pv. in Rice.鉴定一个新型半显性斑点叶突变体，提高了对水稻 pv. 的抗性。
Int J Mol Sci. 2018 Nov 27;19(12):3766. doi: 10.3390/ijms19123766.
3
Characterization and fine mapping of a light-dependent leaf lesion mimic mutant 1 in rice.水稻中一个光依赖型类病斑突变体1的鉴定与精细定位
Plant Physiol Biochem. 2015 Dec;97:44-51. doi: 10.1016/j.plaphy.2015.09.001. Epub 2015 Sep 12.
4
Disruption of EARLY LESION LEAF 1, encoding a cytochrome P450 monooxygenase, induces ROS accumulation and cell death in rice.编码细胞色素P450单加氧酶的早期病变叶片1发生破坏，会诱导水稻体内活性氧积累和细胞死亡。
Plant J. 2021 Feb;105(4):942-956. doi: 10.1111/tpj.15079. Epub 2020 Dec 20.
5
The germin-like protein OsGLP2-1 enhances resistance to fungal blast and bacterial blight in rice.类胚蛋白 OsGLP2-1 增强水稻对真菌性枯萎病和细菌性条斑病的抗性。
Plant Mol Biol. 2016 Nov;92(4-5):411-423. doi: 10.1007/s11103-016-0521-4. Epub 2016 Sep 15.
6
The rice (Oryza sativa) blast lesion mimic mutant, blm, may confer resistance to blast pathogens by triggering multiple defense-associated signaling pathways.水稻（Oryza sativa）稻瘟病病斑模拟突变体blm可能通过触发多种防御相关信号通路来赋予对稻瘟病菌的抗性。
Plant Physiol Biochem. 2005 Apr;43(4):397-406. doi: 10.1016/j.plaphy.2005.03.002. Epub 2005 Apr 7.
7
Loss-of-function mutations of OsbHLH044 transcription factor lead to salinity sensitivity and a greater chalkiness in rice (Oryza sativa L.).OsbHLH044 转录因子功能丧失突变导致水稻（Oryza sativa L.）的耐盐性和垩白度增加。
Plant Physiol Biochem. 2022 Dec 15;193:110-123. doi: 10.1016/j.plaphy.2022.10.033. Epub 2022 Nov 3.
8
A rice calcium-dependent protein kinase OsCPK12 oppositely modulates salt-stress tolerance and blast disease resistance.一个水稻钙依赖蛋白激酶 OsCPK12 相反地调节耐盐性和稻瘟病抗性。
Plant J. 2012 Jan;69(1):26-36. doi: 10.1111/j.1365-313X.2011.04766.x. Epub 2011 Oct 10.
9
CRISPR/Cas9-mediated knockout of Bsr-d1 enhances the blast resistance of rice in Northeast China.CRISPR/Cas9 介导的 Bsr-d1 基因敲除增强了东北地区水稻的抗爆裂性。
Plant Cell Rep. 2024 Mar 18;43(4):100. doi: 10.1007/s00299-024-03192-0.
10
OsNBL3, a mitochondrion-localized pentatricopeptide repeat protein, is involved in splicing nad5 intron 4 and its disruption causes lesion mimic phenotype with enhanced resistance to biotic and abiotic stresses.OsNBL3，一种定位于线粒体的五肽重复蛋白，参与了 nad5 内含子 4 的剪接，其缺失导致了损伤模拟表型，增强了对生物和非生物胁迫的抗性。
Plant Biotechnol J. 2021 Nov;19(11):2277-2290. doi: 10.1111/pbi.13659. Epub 2021 Jul 17.

引用本文的文献

1
Lesion Mimic Mutant: An Ideal Genetic Material for Deciphering the Balance Between Plant Immunity and Growth.类病变突变体：解析植物免疫与生长平衡的理想遗传材料。
Rice (N Y). 2025 May 13;18(1):34. doi: 10.1186/s12284-025-00789-1.
2
Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.CRISPR技术在植物基因编辑中用于提高对生物因子耐受性的应用：一项系统综述
Curr Issues Mol Biol. 2024 Oct 2;46(10):11086-11123. doi: 10.3390/cimb46100659.
3
Genome-Wide Identification of Catalase Gene Family and the Function of in Eggplant Response to Salt Stress.

本文引用的文献

1
A putative SUBTILISIN-LIKE SERINE PROTEASE 1 (SUBSrP1) regulates anther cuticle biosynthesis and panicle development in rice.一个假定的 SUBTILISIN-LIKE SERINE PROTEASE 1（SUBSrP1）调节水稻花粉囊表皮生物合成和穗发育。
J Adv Res. 2022 Dec;42:273-287. doi: 10.1016/j.jare.2022.01.003. Epub 2022 Jan 8.
2
Fine-Tuning Roles of Osa-miR159a in Rice Immunity Against Magnaporthe oryzae and Development.微调Osa-miR159a在水稻抗稻瘟病免疫及发育中的作用
Rice (N Y). 2021 Mar 6;14(1):26. doi: 10.1186/s12284-021-00469-w.
3
Disruption of EARLY LESION LEAF 1, encoding a cytochrome P450 monooxygenase, induces ROS accumulation and cell death in rice.
基因组-wide 鉴定过氧化氢酶基因家族和在茄子响应盐胁迫中的功能。
Int J Mol Sci. 2023 Nov 30;24(23):16979. doi: 10.3390/ijms242316979.
4
Rice β-Glucosidase 4 (Os1βGlu4) Regulates the Hull Pigmentation via Accumulation of Salicylic Acid.水稻β-葡萄糖苷酶 4（Os1βGlu4）通过积累水杨酸调节种皮色素沉着。
Int J Mol Sci. 2022 Sep 13;23(18):10646. doi: 10.3390/ijms231810646.
编码细胞色素P450单加氧酶的早期病变叶片1发生破坏，会诱导水稻体内活性氧积累和细胞死亡。
Plant J. 2021 Feb;105(4):942-956. doi: 10.1111/tpj.15079. Epub 2020 Dec 20.
4
Ferroptosis contributes to developmental cell death in rice blast.铁死亡参与稻瘟病中的发育性细胞死亡。
New Phytol. 2020 Sep;227(6):1831-1846. doi: 10.1111/nph.16636. Epub 2020 Jun 9.
5
Engineering a New Chloroplastic Photorespiratory Bypass to Increase Photosynthetic Efficiency and Productivity in Rice.工程化新型叶绿体光呼吸旁路以提高水稻光合作用效率和生产力。
Mol Plant. 2019 Feb 4;12(2):199-214. doi: 10.1016/j.molp.2018.11.013. Epub 2019 Jan 10.
6
OsACL-A2 negatively regulates cell death and disease resistance in rice.OsACL-A2 负调控水稻细胞死亡和抗病性。
Plant Biotechnol J. 2019 Jul;17(7):1344-1356. doi: 10.1111/pbi.13058. Epub 2019 Jan 10.
7
Mutation in Rice Results in Cell Death, Enhanced Disease-Resistance, Altered Seed Dormancy and Development.水稻中的突变导致细胞死亡、抗病性增强、种子休眠和发育改变。
Front Plant Sci. 2018 Mar 28;9:405. doi: 10.3389/fpls.2018.00405. eCollection 2018.
8
Excessive UDPG resulting from the mutation of UAP1 causes programmed cell death by triggering reactive oxygen species accumulation and caspase-like activity in rice.由于 UAP1 突变导致 UDPG 过量，从而在水稻中通过触发活性氧积累和半胱天冬酶样活性引发程序性细胞死亡。
New Phytol. 2018 Jan;217(1):332-343. doi: 10.1111/nph.14818. Epub 2017 Oct 2.
9
CRISPR-GE: A Convenient Software Toolkit for CRISPR-Based Genome Editing.CRISPR-GE：一款用于基于CRISPR的基因组编辑的便捷软件工具包。
Mol Plant. 2017 Sep 12;10(9):1246-1249. doi: 10.1016/j.molp.2017.06.004. Epub 2017 Jun 15.
10
Novel mutant alleles of the starch synthesis gene TaSSIVb-D result in the reduction of starch granule number per chloroplast in wheat.淀粉合成基因TaSSIVb-D的新型突变等位基因导致小麦每个叶绿体中淀粉粒数量减少。
BMC Genomics. 2017 May 8;18(1):358. doi: 10.1186/s12864-017-3724-4.