人成纤维细胞对贝诺福奥噻嗪的体外耐受性。
Tolerance of Human Fibroblasts to Benfo-Oxythiamine In Vitro.
机构信息
Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.
Department of Oral and Maxillofacial Surgery, Guiyang Hospital of Stomatology, Guiyang 050017, China.
出版信息
Int J Environ Res Public Health. 2022 Mar 30;19(7):4112. doi: 10.3390/ijerph19074112.
OBJECTIVES
To explore the potential application of B-OT in the aspiration tract.
MATERIALS AND METHODS
We conceived and optimized an in vitro model simulating the mouth-washing process to assess tolerance to B-OT on primary human gingival fibroblasts. Cells derived from 4 unrelated donors were flushed with medium containing drugs of various concentration for one minute twice daily for 3 days.
RESULTS
No effect was seen on the cells up to 1000 µM B-OT. In addition, we treated the cells with B-OT permanently in medium, corresponding to a systemic treatment. No effect was seen by 10 µM B-OT and only a slight reduction (approximately 10%) was seen by 100 µM B-OT.
CONCLUSIONS
Our results suggest good tolerance of oral cells for B-OT, favoring the further development of this antiviral reagent as a mouth-washing solution and nasal spray.
目的
探索 B-OT 在吸引道中的潜在应用。
材料和方法
我们构思并优化了一种体外模拟漱口过程的模型，以评估原发性人牙龈成纤维细胞对 B-OT 的耐受性。来自 4 个无关供体的细胞用含有不同浓度药物的培养基冲洗，每天两次，每次 1 分钟，持续 3 天。
结果
高达 1000µM 的 B-OT 对细胞没有影响。此外，我们用 B-OT 对细胞进行了永久性的介质处理，相当于全身治疗。10µM 的 B-OT 没有效果，而 100µM 的 B-OT 只有轻微的减少（约 10%）。
结论
我们的结果表明口腔细胞对 B-OT 具有良好的耐受性，这有利于进一步开发这种抗病毒试剂作为漱口液和鼻腔喷雾剂。
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