Nadiminti Sravanthi S P, Koushika Sandhya P
Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India.
Methods Mol Biol. 2022;2431:499-530. doi: 10.1007/978-1-0716-1990-2_27.
Axonal transport is an essential component of neuronal function. Several neurodegenerative disorders have been associated with defects in cargo transport. Thus, studying axonal transport is important to understand such disorders. Live imaging of fluorescently labeled cargo is a prevailing technique to study properties of axonal transport. C. elegans is both transparent and genetically amenable, making it an excellent model system to study axonal transport. In this chapter, we describe protocols to live image several neuronal cargo in vivo in C. elegans neurons.
轴突运输是神经元功能的重要组成部分。几种神经退行性疾病与货物运输缺陷有关。因此，研究轴突运输对于理解此类疾病很重要。对荧光标记货物进行实时成像，是研究轴突运输特性的一种常用技术。秀丽隐杆线虫既透明又易于进行基因操作，使其成为研究轴突运输的极佳模型系统。在本章中，我们描述了在秀丽隐杆线虫神经元中对几种神经元货物进行体内实时成像的实验方案。
