Snella M C
Br J Exp Pathol. 1986 Dec;67(6):801-7.
Alveolar macrophages (AM) isolated from normal guinea-pigs and from those chronically exposed to endotoxin (LPS) were cultured in the presence of various concentrations of LPS (from 0.5 ng to 5 micrograms/ml). The presence of a neutrophil chemotactic factor (NCF) in culture supernatants was tested in migrations chambers. Contamination of all reagents has been tested using LAL test (Limulus Amoebocyte Lysate). The results indicate a production of NCF with low LPS concentrations (0.5 and 5 ng/ml) within the first 6 h of incubation: when larger doses are used the response decreases and a significant inhibition is observed with 5 micrograms LPS/ml (P less than or equal to 0.05). When contaminated medium was used, all responses observed were three times higher than with LPS-free medium (P less than or equal to 0.01). However, the response pattern was the same. AM from chronically exposed animals exhibit the same response patterns: the magnitude of NCF production was higher than with normal AM but not significantly. The data suggests that initial conditions of AM in vitro or in vivo with reference to LPS contamination have to be determined as they are of importance when AM NCF production has to be tested.
从正常豚鼠以及长期暴露于内毒素（脂多糖，LPS）的豚鼠中分离出肺泡巨噬细胞（AM），并在存在不同浓度LPS（0.5纳克至5微克/毫升）的情况下进行培养。在迁移小室中检测培养上清液中是否存在中性粒细胞趋化因子（NCF）。使用鲎试剂试验（鲎变形细胞溶解物）检测所有试剂是否被污染。结果表明，在孵育的最初6小时内，低浓度LPS（0.5和5纳克/毫升）可诱导NCF产生：当使用较大剂量时，反应减弱，且在5微克LPS/毫升时观察到显著抑制（P≤0.05）。当使用受污染的培养基时，观察到的所有反应比无LPS培养基高出三倍（P≤0.01）。然而，反应模式相同。来自长期暴露动物的AM表现出相同的反应模式：NCF产生的幅度高于正常AM，但无显著差异。数据表明，在测试AM的NCF产生时，必须确定AM在体外或体内关于LPS污染的初始条件，因为它们很重要。
