Havell E A, Spitalny G L
J Reticuloendothel Soc. 1983 May;33(5):369-80.
Pure cultures of murine bone marrow-derived macrophages produce interferon (IFN) after exposure to endotoxin. The levels of endotoxin-induced IFN were enhanced 5- to 20-fold by pretreating (priming) macrophages with murine interferons produced by either NDV-induced L cells, which consisted of a mixture of IFN alpha and IFN beta, or IFN gamma produced by spleen cells stimulated with phytohemagglutinin. Studies conducted on the kinetics of IFN release from endotoxin-induced macrophages demonstrated that peak synthesis occurred within 2-4 hr and was completed 6 hr after the start of treatment. The addition of actinomycin D to macrophages, up to 1 hr after exposure to endotoxin, completely inhibited release of interferon, thus indicating that gene transcription was required for interferon synthesis. The inclusion of cycloheximide in the medium of endotoxin or Poly(I) X Poly(C)-induced macrophages, although inhibiting 90% of protein synthesis, resulted in a superinducing effect, in that induced macrophages treated with cycloheximide produced higher levels of IFN than macrophages not treated with the inhibitor of protein synthesis. Antigenic characterization of macrophage IFNs revealed that endotoxin-induced IFN was neutralized to a higher degree than virus-induced IFNs derived from either macrophages or L cells.
源自小鼠骨髓的巨噬细胞纯培养物在接触内毒素后会产生干扰素（IFN）。通过用由新城疫病毒（NDV）诱导的L细胞产生的小鼠干扰素（由IFNα和IFNβ的混合物组成）或用植物血凝素刺激的脾细胞产生的IFNγ对巨噬细胞进行预处理（致敏），内毒素诱导的IFN水平可提高5至20倍。对内毒素诱导的巨噬细胞释放IFN的动力学研究表明，峰值合成在2至4小时内发生，并在处理开始后6小时完成。在内毒素暴露后长达1小时向巨噬细胞中添加放线菌素D，可完全抑制干扰素的释放，这表明干扰素合成需要基因转录。在内毒素或聚肌苷酸-聚胞苷酸（Poly(I) X Poly(C)）诱导的巨噬细胞培养基中加入环己酰亚胺，尽管抑制了90%的蛋白质合成，但却产生了超诱导作用，即经环己酰亚胺处理的诱导巨噬细胞产生的IFN水平高于未用蛋白质合成抑制剂处理的巨噬细胞。巨噬细胞IFN的抗原特性表明，内毒素诱导的IFN比源自巨噬细胞或L细胞的病毒诱导的IFN被中和的程度更高。
