Benkö S, Hilkmann H, Vigh L, van Blitterswijk W J
Biochim Biophys Acta. 1987 Jan 26;896(2):129-35. doi: 10.1016/0005-2736(87)90173-8.
Optimal reaction conditions were established for hydrogenation of plasma membranes of living murine GRSL leukemia cells, using the water-soluble catalyst Pd(QS)2 (QS, sulphonated alizarine; C14H6O7NaS). Under these conditions more than 80% of the cells remained viable. Analysis by gas chromatography revealed that hydrogenation occurred predominantly in the 18:2, 20:4 and 22:6 fatty acyl chains of the membrane phospholipids. Under the same conditions hydrogenation was also performed in purified plasma membranes from GRSL cells and from rat liver, and in liposomes prepared from the total lipid extracts of these membranes. Hydrogenation increased the lipid structural order parameter in the membranes, as measured by fluorescence polarization. This increase was more pronounced in the liposomes (46%) than in the plasma membranes (17-25%). Hydrogenation increased the expression of a 15 kDa antigen on the surface of viable GRSL cells, as measured in a Fluorescence Activated Cell Sorter, using monoclonal antibodies. The expression of four other antigens, among which H-2k, was not or much less affected by this treatment.
使用水溶性催化剂Pd(QS)2（QS，磺化茜素；C14H6O7NaS），确定了活鼠GRSL白血病细胞质膜氢化的最佳反应条件。在这些条件下，超过80%的细胞仍保持活力。气相色谱分析表明，氢化主要发生在膜磷脂的18:2、20:4和22:6脂肪酸酰基链中。在相同条件下，也对来自GRSL细胞和大鼠肝脏的纯化质膜以及由这些膜的总脂质提取物制备的脂质体进行了氢化。通过荧光偏振测量，氢化增加了膜中的脂质结构有序参数。这种增加在脂质体中（46%）比在质膜中（17 - 25%）更明显。使用单克隆抗体，通过荧光激活细胞分选仪测量，氢化增加了存活的GRSL细胞表面15 kDa抗原的表达。其他四种抗原的表达，其中包括H - 2k，不受该处理影响或受影响较小。
