Anderson K C, Ritz J, Takvorian T, Coral F, Daley H, Gorgone B C, Freedman A S, Canellos G P, Schlossman S F, Nadler L M
Blood. 1987 Feb;69(2):597-604.
Hematologic engraftment and immune reconstitution were examined in patients who received cyclophosphamide and total body irradiation therapy followed by infusion of autologous bone marrow purged with anti-B1 monoclonal antibody (MoAb) and complement as therapy for non-Hodgkin's lymphoma. Hematologic engraftment was prompt with return of greater than or equal to 0.5 X 10(3)/microL granulocytes and greater than or equal to 2 X 10(4)/microL platelets at a median of 26 and 29 days posttransplant, respectively. Immunologic reconstitution, in contrast, was prolonged. Normal numbers of circulating B cells were consistently noted by five months posttransplant, whereas return of normal immunoglobulin levels in some patients did not occur for one year. Normal numbers of T cells were evident within the first month posttransplant, but a reversed T4:T8 ratio persisted in some patients up to three years. In vitro responses of either B cells to triggers of activation or of T cells to mitogens and antigens were not normal for at least three months posttransplant. Natural killer (NK) cells predominated early after transplant and may demonstrate cytotoxicity against tumor cells. Our studies demonstrate that transplantation with anti-B1 purged autologous bone marrow results in complete hematologic and delayed immunologic engraftment. No significant acute or chronic clinical toxicities have been observed.
对接受环磷酰胺和全身照射治疗，随后输注用抗B1单克隆抗体（MoAb）和补体清除的自体骨髓作为非霍奇金淋巴瘤治疗方法的患者，进行了血液学植入和免疫重建检查。血液学植入迅速，在移植后中位时间分别为26天和29天时，粒细胞恢复至大于或等于0.5×10³/微升，血小板恢复至大于或等于2×10⁴/微升。相比之下，免疫重建则有所延迟。移植后五个月时持续观察到循环B细胞数量正常，而一些患者的免疫球蛋白水平在一年内未恢复正常。移植后第一个月内T细胞数量正常，但一些患者的T4:T8比值倒置持续长达三年。移植后至少三个月内，B细胞对激活触发因素的体外反应或T细胞对有丝分裂原和抗原的体外反应均不正常。自然杀伤（NK）细胞在移植后早期占主导地位，可能对肿瘤细胞表现出细胞毒性。我们的研究表明，用抗B1清除的自体骨髓进行移植可导致完全的血液学植入和延迟的免疫植入。未观察到明显的急性或慢性临床毒性。
