未分化牛乳腺上皮细胞体外增殖模型。
A model for in vitro proliferation of undifferentiated bovine mammary epithelial cells.
作者信息
Shamay A, Gertler A
出版信息
Cell Biol Int Rep. 1986 Dec;10(12):923-9. doi: 10.1016/0309-1651(86)90112-8.
PMID:3542240
Abstract
A method for culturing bovine undifferentiated mammary epithelial cells embedded in collagen gels is described. Cell growth was quantitated by incorporation of tritiated thymidine. Maximal rate of incorporation was achieved in media supplemented with sera, however considerable growth was observed in serum-free medium supplemented with insulin, transferrin and selenium. The growth promoting effect of insulin was maximally expressed only at high nonphysiological concentrations.
摘要
描述了一种培养包埋在胶原凝胶中的牛未分化乳腺上皮细胞的方法。通过掺入氚标记的胸腺嘧啶核苷来定量细胞生长。在补充有血清的培养基中实现了最大掺入率，然而在补充有胰岛素、转铁蛋白和硒的无血清培养基中观察到了相当程度的生长。胰岛素的生长促进作用仅在高非生理浓度下才最大程度地表现出来。
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