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将核酸电路与CRISPR-Cas12a系统相结合用于通用型和信号开启检测。

Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection.

作者信息

Zhao Rujian, Yu Chunxu, Lu Baiyang, Li Bingling

机构信息

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun Jilin 130022 China

School of Applied Chemistry and Engineering, University of Science and Technology of China Hefei Anhui 230026 China.

出版信息

RSC Adv. 2022 Apr 4;12(17):10374-10378. doi: 10.1039/d2ra01332k. eCollection 2022 Mar 31.

DOI:10.1039/d2ra01332k
PMID:35425009
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8977996/
Abstract

We report a universal and signal-on HCR based detection platform innovatively coupling the CRISPR-Cas12a system with HCR. By using this CRISPR-HCR pathway, we can detect different targets by only changing the crRNA. The CRISPR-HCR platform coupling with an upstream amplifier can achieve a practical sensitivity as low as ∼aM of ASFV gene in serum.

摘要

我们报告了一种基于杂交链式反应（HCR）的通用且信号开启的检测平台，该平台创新性地将CRISPR-Cas12a系统与HCR耦合。通过使用这种CRISPR-HCR途径，我们仅通过改变crRNA就能检测不同的靶标。与上游放大器耦合的CRISPR-HCR平台在血清中对非洲猪瘟病毒（ASFV）基因可实现低至约aM的实际检测灵敏度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/204b/8977996/ff8059382622/d2ra01332k-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/204b/8977996/d18883ecdbf2/d2ra01332k-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/204b/8977996/46e4550302ab/d2ra01332k-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/204b/8977996/f923a95c92d8/d2ra01332k-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/204b/8977996/ff8059382622/d2ra01332k-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/204b/8977996/d18883ecdbf2/d2ra01332k-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/204b/8977996/46e4550302ab/d2ra01332k-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/204b/8977996/f923a95c92d8/d2ra01332k-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/204b/8977996/ff8059382622/d2ra01332k-f3.jpg

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