• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

色氨酸54在大肠杆菌单链DNA结合蛋白与单链多核苷酸结合中的作用。

Role of tryptophan 54 in the binding of E. coli single-stranded DNA-binding protein to single-stranded polynucleotides.

作者信息

Khamis M I, Casas-Finet J R, Maki A H, Murphy J B, Chase J W

出版信息

FEBS Lett. 1987 Jan 26;211(2):155-9. doi: 10.1016/0014-5793(87)81427-8.

DOI:10.1016/0014-5793(87)81427-8
PMID:3542565
Abstract

Fluorescence and optical detection of triplet state magnetic resonance spectroscopy have been employed to study the complexes formed by single-stranded polynucleotides with both E. coli single-stranded DNA-binding protein and an E. coli ssb gene product in which Trp-54 is replaced by phenylalanine using site specific oligonucleotide mutagenesis. Our results strongly suggest the involvement of Trp-54 in stabilizing the protein-nucleic acid complexes via stacking interactions of the aromatic residue with the nucleotide bases.

摘要

已采用三重态磁共振光谱的荧光和光学检测方法，来研究单链多核苷酸与大肠杆菌单链DNA结合蛋白以及通过位点特异性寡核苷酸诱变使色氨酸-54被苯丙氨酸取代的大肠杆菌单链结合蛋白基因产物所形成的复合物。我们的结果有力地表明，色氨酸-54通过芳香族残基与核苷酸碱基的堆积相互作用参与稳定蛋白质-核酸复合物。

相似文献

1
Role of tryptophan 54 in the binding of E. coli single-stranded DNA-binding protein to single-stranded polynucleotides.色氨酸54在大肠杆菌单链DNA结合蛋白与单链多核苷酸结合中的作用。
FEBS Lett. 1987 Jan 26;211(2):155-9. doi: 10.1016/0014-5793(87)81427-8.
2
Investigation of the role of individual tryptophan residues in the binding of Escherichia coli single-stranded DNA binding protein to single-stranded polynucleotides. A study by optical detection of magnetic resonance and site-selected mutagenesis.大肠杆菌单链DNA结合蛋白与单链多核苷酸结合中单个色氨酸残基作用的研究。通过磁共振光学检测和位点特异性诱变进行的一项研究。
J Biol Chem. 1987 Aug 15;262(23):10938-45.
3
Triplet state properties of tryptophan residues in complexes of mutated Escherichia coli single-stranded DNA binding proteins with single-stranded polynucleotides.突变型大肠杆菌单链DNA结合蛋白与单链多核苷酸复合物中色氨酸残基的三重态性质
Biophys J. 1989 May;55(5):927-36. doi: 10.1016/S0006-3495(89)82891-7.
4
Tryptophan 54 and phenylalanine 60 are involved synergistically in the binding of E. coli SSB protein to single-stranded polynucleotides.色氨酸54和苯丙氨酸60协同参与大肠杆菌单链结合蛋白（SSB蛋白）与单链多核苷酸的结合。
FEBS Lett. 1987 Aug 17;220(2):347-52. doi: 10.1016/0014-5793(87)80844-x.
5
Optically detected magnetic resonance of tryptophan residues in Escherichia coli ssb gene product and E. coli plasmid-encoded single-stranded DNA-binding proteins and their complexes with poly(deoxythymidylic) acid.大肠杆菌单链结合蛋白基因产物和大肠杆菌质粒编码的单链DNA结合蛋白中色氨酸残基的光学检测磁共振及其与聚（脱氧胸苷酸）的复合物。
J Biol Chem. 1987 Jun 25;262(18):8574-83.
6
Optically detected magnetic resonance of tryptophan residues in complexes formed between a bacterial single-stranded DNA binding protein and heavy atom modified poly(uridylic acid).
Biochemistry. 1987 Jun 16;26(12):3347-54. doi: 10.1021/bi00386a015.
7
Stacking interactions of tryptophan residues and nucleotide bases in complexes formed between Escherichia coli single-stranded DNA binding protein and heavy atom-modified poly(uridylic) acid. A study by optically detected magnetic resonance spectroscopy.大肠杆菌单链DNA结合蛋白与重原子修饰的聚（尿苷酸）酸形成的复合物中色氨酸残基与核苷酸碱基的堆积相互作用。光检测磁共振光谱研究。
J Biol Chem. 1987 Feb 5;262(4):1725-33.
8
An IncY plasmid-encoded single-stranded DNA-binding protein from Escherichia coli shows the identical pattern of stacked tryptophan residues as the chromosomal ssb gene product.
Eur J Biochem. 1988 Dec 1;178(1):101-7. doi: 10.1111/j.1432-1033.1988.tb14434.x.
9
Energy transfer in complexes of E. coli single-stranded DNA-binding protein with single-stranded poly-(2-thiouridylic acid).大肠杆菌单链DNA结合蛋白与单链聚（2-硫代尿苷酸）复合物中的能量转移
FEBS Lett. 1990 Feb 26;261(2):389-91. doi: 10.1016/0014-5793(90)80599-e.
10
p10, a low molecular weight single-stranded nucleic acid binding protein of murine leukemia retroviruses, shows stacking interactions of its single tryptophan residue with nucleotide bases.
Biochemistry. 1988 Feb 23;27(4):1172-8. doi: 10.1021/bi00404a016.

引用本文的文献

1
Structure-Function Analysis Reveals the Singularity of Plant Mitochondrial DNA Replication Components: A Mosaic and Redundant System.结构-功能分析揭示了植物线粒体DNA复制组件的独特性：一个镶嵌且冗余的系统。
Plants (Basel). 2019 Nov 21;8(12):533. doi: 10.3390/plants8120533.
2
A stable tetramer is not the only oligomeric state that mitochondrial single-stranded DNA binding proteins can adopt.一种稳定的四聚体并不是线粒体单链 DNA 结合蛋白可采用的唯一寡聚状态。
J Biol Chem. 2019 Mar 15;294(11):4137-4144. doi: 10.1074/jbc.RA118.007048. Epub 2019 Jan 7.
3
Structural Mechanisms of Cooperative DNA Binding by Bacterial Single-Stranded DNA-Binding Proteins.
细菌单链 DNA 结合蛋白协同 DNA 结合的结构机制。
J Mol Biol. 2019 Jan 18;431(2):178-195. doi: 10.1016/j.jmb.2018.11.019. Epub 2018 Nov 22.
4
Dynamic stepwise opening of integron attC DNA hairpins by SSB prevents toxicity and ensures functionality.单链结合蛋白（SSB）介导的整合子attC DNA发夹结构动态逐步打开可防止毒性并确保功能。
Nucleic Acids Res. 2017 Oct 13;45(18):10555-10563. doi: 10.1093/nar/gkx670.
5
Is a fully wrapped SSB-DNA complex essential for Escherichia coli survival?完全包裹的单链结合蛋白 - 脱氧核糖核酸（SSB-DNA）复合物对大肠杆菌的生存至关重要吗？
Nucleic Acids Res. 2016 May 19;44(9):4317-29. doi: 10.1093/nar/gkw262. Epub 2016 Apr 15.
6
Structural dynamics of E. coli single-stranded DNA binding protein reveal DNA wrapping and unwrapping pathways.大肠杆菌单链DNA结合蛋白的结构动力学揭示了DNA包裹和解开途径。
Elife. 2015 Aug 25;4:e08193. doi: 10.7554/eLife.08193.
7
Human single-stranded DNA binding proteins are essential for maintaining genomic stability.人类单链 DNA 结合蛋白对于维持基因组稳定性至关重要。
BMC Mol Biol. 2013 Apr 1;14:9. doi: 10.1186/1471-2199-14-9.
8
Physical and functional interactions between human mitochondrial single-stranded DNA-binding protein and tumour suppressor p53.人类线粒体单链DNA结合蛋白与肿瘤抑制因子p53之间的物理和功能相互作用。
Nucleic Acids Res. 2009 Feb;37(2):568-81. doi: 10.1093/nar/gkn974. Epub 2008 Dec 9.
9
Triplet state sublevel kinetics of tryptophan 54 in the complex of Escherichia coli single-stranded DNA binding protein with single-stranded poly(deoxythymidylic) acid.大肠杆菌单链DNA结合蛋白与单链聚（脱氧胸苷酸）酸复合物中色氨酸54的三重态亚能级动力学
Biophys J. 1987 Nov;52(5):867-72. doi: 10.1016/S0006-3495(87)83280-0.
10
Triplet state properties of tryptophan residues in complexes of mutated Escherichia coli single-stranded DNA binding proteins with single-stranded polynucleotides.突变型大肠杆菌单链DNA结合蛋白与单链多核苷酸复合物中色氨酸残基的三重态性质
Biophys J. 1989 May;55(5):927-36. doi: 10.1016/S0006-3495(89)82891-7.