Cai Yue, Yuan Binbin, Ma Xiaoyue, Fang Guodong, Zhou Dongmei, Gao Juan
Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Sci Total Environ. 2022 Aug 10;833:155258. doi: 10.1016/j.scitotenv.2022.155258. Epub 2022 Apr 13.
Nano-enabled foliar-application could be an ideal strategy for advancing agricultural productivity. However, it remains largely unknown whether they inhibit or promote the uptake of pollutants. Here, we systematically examined how foliar applying SiO nanoparticles (nSiO) and ZnO nanoparticles (nZnO) (20 nm, 100 mg·L), influence polycyclic aromatic hydrocarbons (PAHs) uptake in 4-week-old amaranth (Amaranthus tricolor L.). Results showed that foliar application of nSiO or nZnO enhanced amaranth biomass by 20.2-26.4% but decreased PAHs bioaccumulation in leaves by 20.4-54.9% after 7-d incubation. Changes regarding amino acid-related pathways (alanine/aspartate/glutamate metabolism and arginine biosynthesis) and energy maintenance pathways (TCA cycle) were observed in amaranth leaves after foliar application of nSiO and nZnO. Specific PLS-DA analyses with total PAHs uptake as the biological endpoint showed that the contents of PAHs positively correlated with valine (R = 0.799) and tyrosine (R = 0.789), but negatively correlated with D-tagatose (R = 0.805) and L-gulonolactone (R = 0.877), indicating greater oxidant stress under higher PAHs level. We propose that mechanisms of declined uptake of PAHs involve the biomass-dependent dilute effect and activation of biological response against PAHs accumulation. These findings provide a prospective vision on how nano-enabled foliar-application alleviates PAH-enriched environmental burden while producing higher-yield agricultural products, especially for low toxic and biocompatible nSiO.
纳米叶面喷施可能是提高农业生产力的理想策略。然而，它们对污染物吸收的抑制或促进作用在很大程度上仍不清楚。在此，我们系统地研究了叶面喷施二氧化硅纳米颗粒（nSiO）和氧化锌纳米颗粒（nZnO）（20纳米，100毫克·升）如何影响4周龄苋菜（Amaranthus tricolor L.）对多环芳烃（PAHs）的吸收。结果表明，叶面喷施nSiO或nZnO使苋菜生物量提高了20.2-26.4%，但在7天培养后，叶片中PAHs的生物积累量降低了20.4-54.9%。叶面喷施nSiO和nZnO后，苋菜叶片中观察到与氨基酸相关途径（丙氨酸/天冬氨酸/谷氨酸代谢和精氨酸生物合成）和能量维持途径（三羧酸循环）的变化。以总PAHs吸收为生物学终点的特定偏最小二乘判别分析（PLS-DA）表明，PAHs含量与缬氨酸（R = 0.799）和酪氨酸（R = 0.789）呈正相关，但与D-塔格糖（R = 0.805）和L-古洛糖酸内酯（R = 0.877）呈负相关，表明在较高PAHs水平下氧化应激更大。我们认为，PAHs吸收下降的机制涉及生物量依赖性稀释效应和针对PAHs积累的生物反应激活。这些发现为纳米叶面喷施如何在生产高产农产品的同时减轻富含PAH的环境负担提供了前瞻性的见解，特别是对于低毒且具有生物相容性的nSiO。
