Institute of Biochemistry, University of Münster, Germany.
European Institute for Molecular Imaging (EIMI), University of Münster, Germany; Department of Nuclear Medicine, University Hospital Münster, Germany.
Curr Opin Chem Biol. 2022 Jun;68:102144. doi: 10.1016/j.cbpa.2022.102144. Epub 2022 Apr 14.
Inflammation is a common, fast, and innate response of the immune system to sterile or infectious tissue damage or autoimmune triggers. It aims at minimizing tissue destruction and maintaining organ function, hence is vital to life. Therefore, the immune system comprises the concerted action of a variety of different immune cells with specific tasks in the initiation, maintenance, and termination of inflammation. Visualizing their localization, trafficking, and interaction is of utmost importance to unravel the dynamics of inflammation in the living organism and requires tools for cell-specific labeling and imaging. Many concepts for covalent cell-type or protein-specific labeling have been developed, but only few have been implemented for labeling immune cells. Here, we review approaches that were already successful for fluorescent reporters and radioactive nuclides. We also provide a glimpse on emerging technologies that bear potential for immune cell labeling and imaging in vivo.
炎症是免疫系统对无菌或感染性组织损伤或自身免疫触发的常见、快速和固有反应。它旨在最大程度地减少组织破坏并维持器官功能，因此对生命至关重要。因此，免疫系统由各种具有特定任务的不同免疫细胞的协同作用组成，用于炎症的启动、维持和终止。可视化它们的定位、迁移和相互作用对于揭示活生物体中炎症的动态至关重要，这需要用于细胞特异性标记和成像的工具。已经开发出许多用于共价细胞类型或蛋白质特异性标记的概念，但只有少数已用于标记免疫细胞。在这里，我们回顾了已经成功用于荧光报告基因和放射性核素的方法。我们还展望了一些新兴技术，这些技术有可能用于体内免疫细胞标记和成像。
