Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada.
Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.
Immunol Rev. 2022 Mar;306(1):244-257. doi: 10.1111/imr.13040. Epub 2021 Nov 23.
Highly dynamic immune responses are generated toward pathogens or injuries, in vivo. Multiple immune cell types participate in various facets of the response which leads to a concerted effort in the removal and clearance of pathogens or injured tissue and a return to homeostasis. Intravital microscopy (IVM) has been extensively utilized to unravel the dynamics of immune responses, visualizing immune cell behavior in intact living tissues, within a living organism. For instance, the phenomenon of leukocyte recruitment cascade. Importantly, IVM has led to a deep appreciation that immune cell behavior and responses in individual organs are distinct, but also can influence one another. In this review, we discuss how IVM as a tool has been used to study the innate immune responses in various tissues during homeostasis, injury, and infection.
在体内，针对病原体或损伤会产生高度动态的免疫反应。多种免疫细胞类型参与反应的各个方面，导致共同努力清除病原体或受损组织，并恢复体内平衡。活体显微镜 (IVM) 已被广泛用于揭示免疫反应的动态，在活体组织中可视化免疫细胞的行为，在活体生物中。例如，白细胞募集级联现象。重要的是，IVM 使人们深刻认识到，单个器官中免疫细胞的行为和反应是不同的，但也可以相互影响。在这篇综述中，我们讨论了作为一种工具的 IVM 如何用于研究在体内平衡、损伤和感染期间各种组织中的先天免疫反应。
