Shanghai Key Laboratory of Multiphase Materials Chemical Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, People's Republic of China.
Shanghai Key Laboratory of Multiphase Materials Chemical Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, People's Republic of China.
J Colloid Interface Sci. 2022 Aug 15;620:322-332. doi: 10.1016/j.jcis.2022.04.030. Epub 2022 Apr 9.
Designing rational carriers for protein encapsulation and delivery is of great interest in a wide range of applications. Herein, we develop a type of PEGylated polyelectrolyte nanogel that enables efficient protein loading, protection and intracellular delivery. Our design relies on a one pot process involving cooperative electrostatic assembly and polymerization, which constructs polyion complex (PIC) micelles containing cross-linked cationic PDMAEMA network associated with anionic PAA. Further removing PAA chains leads to core-shell nanogels with a cationic network surrounded by poly (ethylene glycol) (PEG) blocks. The established strategy allows to fabricate well-defined nanogels with regulated size and swelling ability depending on pH, salt concentration and cross-link degree. More notably, the abundant positive charges and the thermo-response of PDMAEMA network realize sufficient encapsulation and protection of BSA proteins, while the PEG blocks endow appreciable biocompatibility and successful intracellular protein delivery. Our study establishes a facile and robust approach for preparing core-shell polyelectrolyte nanogels with regulated size and properties, as well as prominent capabilities for protein encapsulation and delivery.
设计用于蛋白质包封和递送的合理载体在广泛的应用中具有重要意义。在此，我们开发了一种聚乙二醇化的聚电解质纳米凝胶，能够有效负载、保护和细胞内递蛋白质。我们的设计依赖于一锅法工艺，涉及协同静电组装和聚合，构建含有交联阳离子 PDMAEMA 网络与阴离子 PAA 的聚离子复合物（PIC）胶束。进一步去除 PAA 链会导致具有阳离子网络的核壳纳米凝胶，其周围是聚（乙二醇）（PEG）块。所建立的策略允许根据 pH 值、盐浓度和交联度来制备具有规定尺寸和溶胀能力的良好定义的纳米凝胶。更值得注意的是，PDMAEMA 网络的丰富正电荷和温度响应实现了对 BSA 蛋白质的充分包封和保护，而 PEG 块赋予了相当的生物相容性和成功的细胞内蛋白质递送。我们的研究建立了一种简便而强大的方法来制备具有规定尺寸和性能的核壳型聚电解质纳米凝胶，以及用于蛋白质包封和递送的突出能力。
