Guo Xiaotong, Lv Min, Li Jinhua, Ding Jing, Wang Yunqing, Fu Longwen, Sun Xiyan, Han Xiaoyue, Chen Lingxin
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China.
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China.
J Hazard Mater. 2022 Jul 15;434:128874. doi: 10.1016/j.jhazmat.2022.128874. Epub 2022 Apr 6.
As a ubiquitous emerging pollutant, microplastics (MPs) have attracted widespread attention. At this stage, researchers mainly employed commercial MPs (CMPs) as the model particles to explore the toxic effects of MPs. But whether CMPs can reflect the effects of realistic MPs (RMPs) still remains unknown. Herein, the effects of commercial and realistic polystyrene MPs on gut microbiota of zebrafish were compared. Considering MPs co-exist with antibiotics in real environment, we further distinguished the effects of CMPs and RMPs when they co-existed with enrofloxacin (ENR). The results revealed that while both CMPs and RMPs significantly shifted the gut microbiota, CMPs exhibited stronger toxic effects and more severe damage to gut. Furthermore, ENR exhibited a distinct effect with both CMPs and RMPs on gut microbiota, while the addition of CMPs and RMPs significantly alleviated the toxicity of ENR. In addition, analysis via Kyoto Encyclopedia of Genes and Genomes pathway database revealed that seven major level 1 pathways associated with metabolism, information processing and diseases in the microbial community were affected. Taken together, this work is the first to report that CMPs could not represent RMPs in terms of toxicity and other behaviors, reminding people the limits of using CMPs in ecotoxicology studies.
作为一种普遍存在的新兴污染物，微塑料（MPs）已引起广泛关注。现阶段，研究人员主要使用商业微塑料（CMPs）作为模型颗粒来探究微塑料的毒性效应。但CMPs能否反映实际微塑料（RMPs）的效应仍不清楚。在此，比较了商业聚苯乙烯微塑料和实际聚苯乙烯微塑料对斑马鱼肠道微生物群的影响。考虑到微塑料在实际环境中与抗生素共存，我们进一步区分了CMPs和RMPs与恩诺沙星（ENR）共存时的影响。结果表明，虽然CMPs和RMPs都显著改变了肠道微生物群，但CMPs表现出更强的毒性效应和对肠道更严重的损伤。此外，ENR对CMPs和RMPs的肠道微生物群均表现出不同的影响，而添加CMPs和RMPs显著减轻了ENR的毒性。此外，通过京都基因与基因组百科全书通路数据库分析发现，微生物群落中与代谢、信息处理和疾病相关的七个主要一级通路受到影响。综上所述，这项工作首次报道了在毒性和其他行为方面CMPs不能代表RMPs，提醒人们在生态毒理学研究中使用CMPs的局限性。
