School of Chemical Engineering and Technology, North University of China, Taiyuan, Shanxi, 030051, China; Shanxi Jingxi Biotechnology Co., Ltd, Taiyuan, Shanxi, 030051, China.
School of Chemical Engineering and Technology, North University of China, Taiyuan, Shanxi, 030051, China.
Plant Physiol Biochem. 2022 Jun 15;181:50-60. doi: 10.1016/j.plaphy.2022.04.001. Epub 2022 Apr 6.
Tree peony (sect. Moutan) is a kind of Traditional Chinese Medicine and ornamental plant, which has been widely cultivated and utilized for thousands of years. To further study the active components of Paeonia ostii (Moutan, Fengdan), six fractions (soluble free (F), soluble esterification, soluble glycosylation, insoluble bound, insoluble esterification and insoluble glycosylation) were extracted from the leaves and roots by alkaline and acid treatment for the first time. Twenty-one typical compounds were identified and quantified by HPLC-MS. The results showed that total phenolic content (TPC) in peony roots (PR) and peony leaves (PL) were as high as 125.48 and 280.38 mg GAE·g dw, which maximizes the extraction efficiency of phenolic compounds, especially leaves, compared with the conventional method. PR-F and PL-F had the highest TPC, antioxidant and anti-tyrosinase activities. Paeoniflorin was the main compounds in PL and PR. It and pentagalloylglucose (PGG) almost reached the anti-tyrosinase level of kojic acid, but they showed different inhibitory mechanisms by molecular docking. On the whole, PR-F, PL-F, PGG and paeoniflorin might be potential for skin whitening products.
牡丹（芍药属牡丹组）是一种中药和观赏植物，已经被广泛种植和利用了数千年。为了进一步研究牡丹凤丹（牡丹、凤丹）的活性成分，首次采用碱性和酸性处理法从其叶和根中提取了六种馏分（可溶游离（F）、可溶酯化、可溶糖基化、不可溶结合、不可溶酯化和不可溶糖基化）。采用 HPLC-MS 鉴定并定量了 21 种典型化合物。结果表明，芍药根（PR）和芍药叶（PL）中的总酚含量（TPC）高达 125.48 和 280.38 mg GAE·g dw，与传统方法相比，最大限度地提高了酚类化合物的提取效率，尤其是叶子。PR-F 和 PL-F 的 TPC、抗氧化和抗酪氨酸酶活性最高。芍药苷是 PL 和 PR 的主要化合物。它和五没食子酰葡萄糖（PGG）几乎达到了曲酸的抗酪氨酸酶水平，但通过分子对接显示出不同的抑制机制。总的来说，PR-F、PL-F、PGG 和芍药苷可能是潜在的皮肤美白产品。
