Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China; Academy of Agricultural Sciences, Southwest University, Chongqing, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Southwest University, Chongqing, China.
Institute of Sericulture Science and Technology Research, Chongqing, China.
Pestic Biochem Physiol. 2022 May;183:105062. doi: 10.1016/j.pestbp.2022.105062. Epub 2022 Feb 24.
Cyetpyrafen belonging to mitochondrial electron transport inhibitors of complex II (METI II) has been widely applied to manage pest mites in China. To investigate the adaption of Tetranychus urticae in the evolution of cyetpyrafen resistance, a study of cross resistance, mode of inheritance and fitness comparison of resistance using indoor cyetpyrafen resistant strain (resistance ratio, RR > 2, 000-fold) was executed. Cyet-R showed serious cross resistance to cyenopyrafen (>2500-fold) and cyflumetofen (~190-fold). The number of resistant genes was evaluated via chi-square (χ) test and the concentration-response curve regarding goodness-of-fit between observed and the expected mortality. The LCs of F (Cyet-R × Tu-YN) and F (Tu-YN × Cyet-R) were 3126.30 mg/L and 2743.97 mg/L, respectively, without significance, suggesting autosomal inheritance. The degree of dominance (D) values of F and F ranged between 0 and 1, revealing an incompletely dominant inheritance in the tested population of Tetranychus urticae. Plots of concentration-response data for the orthogonal backcross and reverse backcross progenies showed a significant deviation from the expected lines, pointing out a polygenic inheritance. Besides, lifetable analysis showed a fitness advantage of Cyet-R with a significantly decreased adult preadult period and significantly increased total fecundity. This study suggested that cyetpyrafen resistance against T. urticae was inherited as autosomal, incompletely dominant and multigenetic and characterized with serious cross resistance and fitness advantage. Therefore, rational application and preventive strategy should be considered to sustain the efficacy of cyetpyrafen against T. urticae.
四氯虫酰胺属于线粒体电子传递抑制剂复合物 II（METI II），已在中国广泛用于防治害螨。为了研究二斑叶螨在抗四氯虫酰胺进化中的适应性，本研究进行了室内抗四氯虫酰胺品系（抗性倍数 RR>2000 倍）的交互抗性、遗传方式和适合度比较。Cyet-R 对 cyenopyrafen（>2500 倍）和 cyflumetofen（~190 倍）表现出严重的交互抗性。通过卡方（χ）检验和基于观察死亡率与预期死亡率拟合度的浓度-反应曲线评估抗性基因的数量。F（Cyet-R×Tu-YN）和 F（Tu-YN×Cyet-R）的 LC 值分别为 3126.30 和 2743.97 mg/L，无显著性差异，表明为常染色体遗传。F 和 F 的显性度（D）值在 0 到 1 之间，表明在测试的二斑叶螨种群中存在不完全显性遗传。正交回交和反向回交后代的浓度-反应数据图显示与预期线显著偏离，表明为多基因遗传。此外，生命表分析表明 Cyet-R 具有适合度优势，成虫前期和总产卵量显著降低。本研究表明，二斑叶螨对四氯虫酰胺的抗性是常染色体、不完全显性和多基因遗传的，具有严重的交互抗性和适合度优势。因此，为了维持四氯虫酰胺对二斑叶螨的防治效果，应考虑合理应用和预防性策略。
