Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna no.2, Karangmalang, Yogyakarta 55281, Indonesia.
Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna no.2, Karangmalang, Yogyakarta 55281, Indonesia.
Vet Parasitol Reg Stud Reports. 2022 May;30:100707. doi: 10.1016/j.vprsr.2022.100707. Epub 2022 Feb 16.
Lymnaeid snails play an essential role in transmitting fasciolosis as intermediate hosts. Therefore, this study aims to use the molecular method to identify liver fluke in lymnaeid snails. A total of 320 lymnaeid snails were collected from a rice field. The samples were dissected to collect cercaria and identified using polymerase chain reaction. Furthermore, the internal transcribed spacer 2 (ITS2) was used as the target gene to identify the species of cercaria. The result showed that 3.75% (12/320) of the snails were infected by Fasciola gigantica, while the phylogenetic tree based on ITS2 showed that the cercaria in this study was monophyletic and similar to species from several countries in Southeast Asia, including China. Furthermore, the haplotype network showed that all four cercaria samples were similar with sequences from several countries. This study suggests that the F. gigantica cercaria isolated from lymnaeid snails in Kulon Progo, Yogyakarta, Indonesia, has a sequence similar to that of other species in Southeast Asian countries, although no hybrid type was detected in these sequences. This is the first report on the molecular identification of cercaria F. gigantica isolated from lymnaeid snails in Yogyakarta, Indonesia.
豆丁螺在作为中间宿主传播片形吸虫病方面起着至关重要的作用。因此，本研究旨在使用分子方法鉴定豆丁螺中的片形吸虫。从稻田中采集了 320 只豆丁螺。对样本进行解剖以收集尾蚴，并使用聚合酶链反应进行鉴定。此外，内部转录间隔区 2（ITS2）被用作鉴定尾蚴种的靶基因。结果显示，3.75%（12/320）的螺被巨片形吸虫感染，而基于 ITS2 的系统发育树显示，本研究中的尾蚴是单系的，与来自东南亚几个国家的物种相似，包括中国。此外，单倍型网络显示，所有四个尾蚴样本与来自几个国家的序列相似。本研究表明，从印度尼西亚日惹省Kulon Progo 的豆丁螺中分离出的巨片形吸虫尾蚴具有与东南亚国家其他物种相似的序列，尽管在这些序列中未检测到杂种类型。这是印度尼西亚日惹首次报道从豆丁螺中分离出的巨片形吸虫尾蚴的分子鉴定。
