Kumar S, Moudgil K D, Band A H, Naraynan P R, Gupta S K, Sharma A K, Talwar G P
Indian J Lepr. 1986 Apr-Jun;58(2):185-90.
A visual dipstick dot enzyme immunoassay (EIA) for diagnosis of leprosy is described. The assay is based on detection of IgM antibodies against phenolic glycolipid (PGL-I) in sera from leprosy patients. The antigen (PGL-I or synthetic disaccharide of PGL-I) was dotted on a nitrocellulose pad stuck on a plastic strip (dipstick). Sera were used at a dilution of 1:200. Peroxidase coupled mouse anti-human IgM monoclonal antibodies were used as the conjugate. A positive test gave a blue dot against a white background. The test was highly specific for leprosy, and was quite sensitive for detection of bacilliferous (BL/LL) leprosy. The antigen dotted and preblocked dipsticks stored at room temperature upto 4 months of observation period, were unable in the assay.
描述了一种用于诊断麻风病的可视试纸条斑点酶免疫测定法（EIA）。该测定法基于检测麻风病患者血清中针对酚糖脂（PGL-I）的IgM抗体。将抗原（PGL-I或PGL-I的合成二糖）点样在粘贴于塑料条（试纸条）上的硝酸纤维素垫上。血清以1:200的稀释度使用。过氧化物酶偶联的小鼠抗人IgM单克隆抗体用作缀合物。阳性检测结果为白色背景上出现蓝色斑点。该检测对麻风病具有高度特异性，对检测有菌型（BL/LL）麻风病相当敏感。在室温下储存长达4个月观察期的点样并预先封闭的试纸条在测定中无法使用。
