催乳素对大鼠脑出血后运动障碍及载脂蛋白E、胶质纤维酸性蛋白和催乳素受体基因表达的影响。
Effects of prolactin on movement disorders and APOE, GFAP, and PRL receptor gene expression following intracerebral hemorrhage in rats.
作者信息
Yousefvand Shiba, Hadjzadeh Mousa-Al-Reza, Keshavarzi Zakieh, Dolatshad Hamid, Vafaee Farzaneh, Mahmoudabady Maryam, Gholamzadeh Virany Zahra
机构信息
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Division of Neuro-Cognitive Sciences, Psychiatry and Behavioural Sciences Research center, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Iran J Basic Med Sci. 2021 Dec;24(12):1709-1716. doi: 10.22038/IJBMS.2021.58176.12927.
OBJECTIVES
Intracerebral hemorrhage (ICH) occurs mostly in the striatum. In ICH, blood prolactin level increases 3-fold. The effects of intracerebroventricular injection (ICV) of prolactin on motor disorders will be investigated.
MATERIALS AND METHODS
This study was performed on 32 male Wistar rats in 4 groups: sham, ICH, and prolactin with 1 μg/2 μl (P1) and 2 μg/2 μl (P2) doses.
RESULTS
The weight of animals on days 1 (˂0.01), 3, and 7 (˂0.05) in the sham and P2 groups increased compared with the ICH group. Neurological Deficit Score (NDS) in ICH and P1 groups decreased, and increased compared with sham and ICH groups (˂0.001), respectively. NDS in the P1 group increased compared with the P2 group on days 1 (˂0.0 5), 3, and 7 (˂0.001). The duration time of rotarod in ICH and P1 groups decreased and increased compared with sham and ICH groups (˂0.001), respectively. The duration time of rotarod in the P1 group on days 3 and 7 increased compared with the P2 group (˂0.001). Travel distance in days 1(˂0.01), 3(˂0.001), and 7(˂0.01) decreased in the ICH group. Prolactin receptor (PRL receptor) expression in ICH, P1, and P2 groups increased compared with sham and ICH groups (˂0.001). Glial fibrillary acidic protein (GFAP) expression (˂0.001) and apolipoprotein E (APOE) (˂0.01) expression in the ICH group increased compared with the sham group. GFAP and APOE expression in the P1 group increased compared with the ICH group (˂0.001). APOE expression in the P1 group increased compared with the P2 group (˂0.001).
CONCLUSION
According to the results, prolactin reduces movement disorders.
目的
脑出血（ICH）大多发生在纹状体。在脑出血中，血液中催乳素水平会增加3倍。将研究脑室内注射（ICV）催乳素对运动障碍的影响。
材料与方法
本研究对32只雄性Wistar大鼠进行，分为4组：假手术组、脑出血组以及剂量为1μg/2μl（P1）和2μg/2μl（P2）的催乳素组。
结果
与脑出血组相比，假手术组和P2组动物在第1天（˂0.01）、第3天和第7天（˂0.05）体重增加。脑出血组和P1组的神经功能缺损评分（NDS）降低，与假手术组和脑出血组相比分别升高（˂0.001）。在第1天（˂0.05）、第3天和第7天（˂0.001），P1组的NDS与P2组相比升高。与假手术组和脑出血组相比，脑出血组和P1组的转棒试验持续时间分别缩短和延长（˂0.001）。在第3天和第7天，P1组的转棒试验持续时间与P2组相比延长（˂0.001）。脑出血组在第1天（˂0.01）、第3天（˂0.001）和第7天（˂0.01）的移动距离缩短。与假手术组和脑出血组相比，脑出血组、P1组和P2组的催乳素受体（PRL受体）表达升高（˂0.001）。与假手术组相比，脑出血组的胶质纤维酸性蛋白（GFAP）表达（˂0.001）和载脂蛋白E（APOE）（˂0.01）表达升高。与脑出血组相比，P1组的GFAP和APOE表达升高（˂0.
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