Tamakoshi Keigo, Ishida Kazuto, Kawanaka Kentaro, Takamatsu Yasuyuki, Tamaki Hiroyuki
Department of Physical Therapy, Niigata University of Health and Welfare, Niigata, Japan; Institute for Human Movement and Medical Sciences, Niigata University of Health and Welfare, Niigata, Japan; Department of Physical Therapy, Nagoya University Graduation School of Medicine, Nagoya, Japan.
Department of Physical Therapy, Nagoya University Graduation School of Medicine, Nagoya, Japan.
J Stroke Cerebrovasc Dis. 2017 Oct;26(10):2232-2239. doi: 10.1016/j.jstrokecerebrovasdis.2017.05.005. Epub 2017 Jun 29.
We investigated the effects of acrobatic training (AT) on expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) subunits in the sensorimotor cortex and striatum after intracerebral hemorrhage (ICH).
Male Wistar rats were divided into 4 groups: ICH without AT (ICH), ICH with AT (ICH + AT), sham operation without AT (SHAM), and sham operation with AT (SHAM + AT). ICH was induced by collagenase injection into the left striatum. The ICH + AT group performed 5 acrobatic tasks daily on days 4-28 post ICH. Forelimb sensorimotor function was evaluated using the forelimb placing test. On days 14 and 29, mRNA expression levels of AMPAR subunits GluR1-4 were measured by real-time reverse transcription-polymerase chain reaction.
Forelimb placing test scores were significantly higher in the ICH + AT group than in the ICH group. Expression levels of all AMPAR subunit mRNAs were significantly higher in the ipsilateral sensorimotor cortex of rats in the ICH + AT group than in that of rats in the ICH group on day 29. GluR3 and GluR4 expression levels were reduced in the ipsilateral striatum of rats in the ICH group compared with that of rats in the SHAM group on day 14.
These changes may play a critical role in motor skills training-induced recovery after ICH.
我们研究了杂技训练（AT）对脑出血（ICH）后感觉运动皮层和纹状体中α-氨基-3-羟基-5-甲基-4-异恶唑丙酸受体（AMPAR）亚基表达的影响。
将雄性Wistar大鼠分为4组：未进行杂技训练的脑出血组（ICH）、进行杂技训练的脑出血组（ICH + AT）、未进行杂技训练的假手术组（SHAM）和进行杂技训练的假手术组（SHAM + AT）。通过向左侧纹状体内注射胶原酶诱导脑出血。ICH + AT组在脑出血后第4至28天每天进行5项杂技任务。使用前肢放置试验评估前肢感觉运动功能。在第14天和第29天，通过实时逆转录-聚合酶链反应测量AMPAR亚基GluR1 - 4的mRNA表达水平。
ICH + AT组的前肢放置试验得分显著高于ICH组。在第29天，ICH + AT组大鼠同侧感觉运动皮层中所有AMPAR亚基mRNA的表达水平均显著高于ICH组大鼠。与SHAM组大鼠相比，ICH组大鼠在第14天时同侧纹状体中GluR3和GluR4的表达水平降低。
这些变化可能在脑出血后运动技能训练诱导的恢复中起关键作用。
