Tong Guangan, Zhang Pingping, Hu Wenbin, Zhang Kun, Chen Xianwen
Department of Neurology, The First Affiliated Hospital of Anhui Medical University, Hefei 230031, Anhui, China.
The Affiliated Hospital of the Neurology Institute, Anhui University of Chinese Medicine, Hefei 230031, Anhui, China.
Parkinsons Dis. 2022 Apr 6;2022:8683877. doi: 10.1155/2022/8683877. eCollection 2022.
Parkinson's disease (PD) is a neurodegenerative disease, a hallmark by the formation of misfolded and aggregated -synuclein proteins. The expression of potential microRNA (miRNA) candidates isolated from serum and cerebrospinal fluid (CSF) exosomes of PD patients was assessed for their diagnostic value and their potential role as biomarkers for PD was explored. In this study, we characterize the expression level of miRNAs in the exosomes of blood sera and cerebrospinal fluid and explore their potential role as biomarkers for PD.
A total of 209 patients having an onset of PD, along with 60 neurodegenerative (ND) disorders and 50 healthy controls were enrolled. Blood samples and CSF samples were collected and exosomes were isolated. The isolated exosomes were characterized using CD63 detection and exosomal RNA was extracted. Serum miRNA profiling was carried out by synthesizing cDNA from the purified RNA and miRNA transcripts were determined by qRT-PCR using SYBR Green PremixScript. microRNA profiling strategy was employed for extracting the exosomal miRNAs from the exosomes.
Five common miRNAs viz. miR-151a-5p, miR-24, mir-485-5p, mir-331-5p, and mir-214 were found to be upregulated with statistical significance in both the serum exosome and CSF exosomes. The investigation revealed that serum and CSF exosomal miRNA molecules are definitive biomarkers for PD with proper specificity and sensitivity.
The significant level of miR-151a-5p, miR-24, mir-485-5p, mir-331-5p, and mir-214 was observed in the serum and CSF which may be established as a biomarker for the diagnosis of PD.
帕金森病（PD）是一种神经退行性疾病，其特征是错误折叠和聚集的α-突触核蛋白的形成。评估了从PD患者血清和脑脊液（CSF）外泌体中分离出的潜在微小RNA（miRNA）候选物的表达，以探讨其诊断价值及其作为PD生物标志物的潜在作用。在本研究中，我们对血清和脑脊液外泌体中miRNA的表达水平进行了表征，并探讨了它们作为PD生物标志物的潜在作用。
共纳入209例新发PD患者，以及60例神经退行性疾病（ND）患者和50名健康对照。采集血样和脑脊液样本并分离外泌体。使用CD63检测对分离出的外泌体进行表征，并提取外泌体RNA。通过从纯化的RNA合成cDNA进行血清miRNA谱分析，并使用SYBR Green PremixScript通过qRT-PCR测定miRNA转录本。采用微小RNA谱分析策略从外泌体中提取外泌体miRNA。
发现5种常见的miRNA，即miR-151a-5p、miR-24、mir-485-5p、mir-331-5p和mir-214在血清外泌体和脑脊液外泌体中均有统计学意义的上调。研究表明，血清和脑脊液外泌体miRNA分子是具有适当特异性和敏感性的PD确定性生物标志物。
在血清和脑脊液中观察到miR-151a-5p、miR-24、mir-485-5p、mir-331-5p和mir-214的显著水平，这可能被确立为诊断PD的生物标志物。
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