Nadia Ahmad Syeda, Raees Ahmad Khawaja, Abdullah Usma, Malik Fiza, Ali Rabiyah, Amir-Ud-Din Umara, Ali Kanwal Muhammad, Inayat Iram
Government Associate College for Women Bhera, District Sargodha, Pakistan.
Department of Zoology, University of Sargodha, District Sargodha, Pakistan.
Int J Reprod Biomed. 2022 Mar 21;20(2):101-110. doi: 10.18502/ijrm.v20i2.10502. eCollection 2022 Mar.
Carbon tetrachloride (CCl ) is extensively used in various industries and induces oxidative stress in humans. Unfortunately, it is one of the neglected potent male reproductive toxicants.
The present research reports the testicular histopathology of CCl and ameliorations by four medicinal fruit pulp extracts (FPEs) in mice.
Sixty male albino mice were divided into six groups (10/group) as per the following: 1. Vehicle control (Vc); 2. CCl (C); 3-6. CCl + (CBa), CCl + (CSn), CCl + (CFc) and CCl + (CGa). Except for the control group, CCl (0.1 mL of 0.2 mL kg ) was given to the animals in corn oil. The four plant extracts (0.1 mL each) were respectively given to the relevant FPE group animals for the next five consecutive days, while the animals in the Vc and CCl groups received water instead of FPE.
The CCl exposure led to various histometric and histological alterations (loss of interstitial tissue and various dislodged tailless spermatids with enlarged heads) that were recovered in all except FPE mice post-treatment. The micrometric data of testicular sections also indicated significant decline in the number of spermatogonia, while the cross-sectional area of the sperm heads remained significantly higher in the CSn and C groups.
Except for ,the three FPEs, especially ,showed rehabilitative properties against CCl exposure-related modifications in testicular histopathologies.
四氯化碳（CCl₄）广泛应用于各种行业，并可在人体内诱发氧化应激。不幸的是，它是一种被忽视的强效男性生殖毒物。
本研究报告了CCl₄对小鼠睾丸的组织病理学影响以及四种药用水果果肉提取物（FPEs）的改善作用。
60只雄性白化小鼠按以下方式分为六组（每组10只）：1. 溶剂对照组（Vc）；2. CCl₄组（C）；3 - 6. CCl₄ + 巴西莓提取物（CBa）组、CCl₄ + 锡兰肉桂提取物（CSn）组、CCl₄ + 番荔枝提取物（CFc）组和CCl₄ + 余甘子提取物（CGa）组。除对照组外，给动物腹腔注射0.1 mL（0.2 mL/kg）溶于玉米油的CCl₄。连续五天分别给相关FPE组动物给予四种植物提取物（各0.1 mL），而Vc组和CCl₄组动物给予水而非FPE。
CCl₄暴露导致各种组织计量学和组织学改变（间质组织丧失以及各种头部增大的无尾精子细胞移位），除CBa FPE组小鼠外，其他组小鼠在治疗后这些改变均得到恢复。睾丸切片的微观测量数据还表明精原细胞数量显著下降，而CSn组和CCl₄组精子头部的横截面积仍显著更高。
除CBa外，三种FPEs，尤其是CSn，对CCl₄暴露相关的睾丸组织病理学改变具有修复特性。
