Lee Ju Hee, Ealey Kafi N, Patel Yash, Verma Navkiran, Thakkar Nikita, Park So Young, Kim Jae-Ryong, Sung Hoon-Ki
Translational Medicine Program, The Hospital for Sick Children, Toronto, ON, Canada.
Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
iScience. 2022 Mar 26;25(4):104166. doi: 10.1016/j.isci.2022.104166. eCollection 2022 Apr 15.
The increased prevalence of obesity and metabolic diseases has heightened interest in adipose tissue biology and its potential as a therapeutic target. To better understand cellular heterogeneity and complexity of white adipose tissue (WAT), we employed cytometry by time-of-flight (CyTOF) to characterize immune and stromal cells in visceral and subcutaneous WAT depots under normal and high-fat diet feeding, by quantifying the expression levels of 32 surface marker proteins. We observed comparable proportions of immune cells in two WAT depots under steady state, but depot-distinct subtypes of adipose precursor cells (APC), suggesting differences in their adipogenic and fibrogenic potential. Furthermore, in addition to pro-inflammatory immune cell shifts, significant pro-fibrotic changes were observed in APCs under high-fat diet, suggesting that APCs are early responders to dietary challenges. We propose CyTOF as a complementary and alternative tool to current high-throughput single-cell transcriptomic analyses to better understand the function and plasticity of adipose tissue.
肥胖和代谢性疾病患病率的增加，引发了人们对脂肪组织生物学及其作为治疗靶点潜力的更大兴趣。为了更好地理解白色脂肪组织（WAT）的细胞异质性和复杂性，我们采用飞行时间流式细胞术（CyTOF），通过量化32种表面标志物蛋白的表达水平，来表征正常饮食和高脂饮食喂养条件下内脏和皮下WAT库中的免疫细胞和基质细胞。我们观察到，在稳态下两个WAT库中免疫细胞比例相当，但脂肪前体细胞（APC）存在库特异性亚型，这表明它们在脂肪生成和纤维化潜力方面存在差异。此外，除了促炎免疫细胞的转变，在高脂饮食条件下，APCs中还观察到显著的促纤维化变化，这表明APCs是饮食挑战的早期响应者。我们提出，CyTOF可作为当前高通量单细胞转录组分析的补充和替代工具，以更好地理解脂肪组织的功能和可塑性。
