Silva Karina Ribeiro, Côrtes Isis, Liechocki Sally, Carneiro João Regis Ivar, Souza Antônio Augusto Peixoto, Borojevic Radovan, Maya-Monteiro Clarissa Menezes, Baptista Leandra Santos
Post-graduation Program of Medical Clinics, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil.
Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology - INMETRO, Duque de Caxias, Rio de Janeiro, Brazil.
PLoS One. 2017 Mar 21;12(3):e0174115. doi: 10.1371/journal.pone.0174115. eCollection 2017.
BACKGROUND/OBJECTIVES: The pathological condition of obesity is accompanied by a dysfunctional adipose tissue. We postulate that subcutaneous, preperitoneal and visceral obese abdominal white adipose tissue depots could have stromal vascular fractions (SVF) with distinct composition and adipose stem cells (ASC) that would differentially account for the pathogenesis of obesity.
In order to evaluate the distribution of SVF subpopulations, samples of subcutaneous, preperitoneal and visceral adipose tissues from morbidly obese women (n = 12, BMI: 46.2±5.1 kg/m2) were collected during bariatric surgery, enzymatically digested and analyzed by flow cytometry (n = 12). ASC from all depots were evaluated for morphology, surface expression, ability to accumulate lipid after induction and cytokine secretion (n = 3).
A high content of preadipocytes was found in the SVF of subcutaneous depot (p = 0.0178). ASC from the three depots had similar fibroblastoid morphology with a homogeneous expression of CD34, CD146, CD105, CD73 and CD90. ASC from the visceral depot secreted the highest levels of IL-6, MCP-1 and G-CSF (p = 0.0278). Interestingly, preperitoneal ASC under lipid accumulation stimulus showed the lowest levels of all the secreted cytokines, except for adiponectin that was enhanced (p = 0.0278).
ASC from preperitoneal adipose tissue revealed the less pro-inflammatory properties, although it is an internal adipose depot. Conversely, ASC from visceral adipose tissue are the most pro-inflammatory. Therefore, ASC from subcutaneous, visceral and preperitoneal adipose depots could differentially contribute to the chronic inflammatory scenario of obesity.
背景/目的：肥胖的病理状况伴随着脂肪组织功能失调。我们推测，皮下、腹膜前和内脏型肥胖腹部白色脂肪组织库可能具有成分不同的基质血管成分（SVF）和脂肪干细胞（ASC），它们在肥胖发病机制中起着不同的作用。
为了评估SVF亚群的分布，在减肥手术期间收集了病态肥胖女性（n = 12，BMI：46.2±5.1 kg/m²）的皮下、腹膜前和内脏脂肪组织样本，进行酶消化并通过流式细胞术分析（n = 12）。对所有脂肪库的ASC进行形态学、表面表达、诱导后脂质积累能力和细胞因子分泌评估（n = 3）。
皮下脂肪库的SVF中前脂肪细胞含量较高（p = 0.0178）。来自三个脂肪库的ASC具有相似的成纤维细胞样形态，CD34、CD146、CD105、CD73和CD90表达均匀。来自内脏脂肪库的ASC分泌的IL-6、MCP-1和G-CSF水平最高（p = 0.0278）。有趣的是，在脂质积累刺激下，腹膜前ASC分泌的所有细胞因子水平最低，但脂联素水平升高（p = 0.0278）。
尽管腹膜前脂肪组织是内部脂肪库，但其ASC的促炎特性较弱。相反，内脏脂肪组织的ASC促炎作用最强。因此，皮下、内脏和腹膜前脂肪库的ASC可能在肥胖的慢性炎症过程中发挥不同作用。
