Department of Cardiovascular Surgery, Second Xiangya Hospital, Central South University.
Hunan Provincial Key Laboratory of Cardiovascular Research.
J Vis Exp. 2022 Mar 30(181). doi: 10.3791/63647.
The key objective of cell therapy after myocardial infarction (MI) is to effectively enhance the cell grafted rate, and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are a promising cell source for cardiac repair after ischemic damage. However, a low grafted rate is a significant obstacle for effective cardiac tissue regeneration after transplantation. This protocol shows that multiple hiPSC-CM ultrasound-guided percutaneous injections into an MI area effectively increase cell transplantation rates. The study also describes the entire hiPSC-CM culture process, pretreatment, and ultrasound-guided percutaneous delivery methods. In addition, the use of human mitochondrial DNA help detect the absence of hiPSC-CMs in other mouse organs. Lastly, this paper describes the changes in cardiac function, angiogenesis, cell size, and apoptosis at the infarcted border zone in mice 4 weeks after cell delivery. It can be concluded that echocardiography-guided percutaneous injection of the left ventricular myocardium is a feasible, relatively invasive, satisfactory, repeatable, and effective cellular therapy.
心肌梗死后细胞治疗的关键目标是有效地提高细胞移植率，人诱导多能干细胞衍生的心肌细胞（hiPSC-CMs）是缺血性损伤后心脏修复的一种很有前途的细胞来源。然而，低移植率是移植后有效进行心脏组织再生的一个重大障碍。本方案表明，将多个 hiPSC-CM 超声引导经皮注射到心肌梗死区域可有效提高细胞移植率。该研究还描述了整个 hiPSC-CM 培养过程、预处理和超声引导经皮传递方法。此外，使用人线粒体 DNA 有助于检测 hiPSC-CMs 在其他小鼠器官中的缺失。最后，本文描述了细胞递送后 4 周小鼠梗死边缘区心功能、血管生成、细胞大小和细胞凋亡的变化。可以得出结论，超声心动图引导的左心室心肌经皮注射是一种可行、相对微创、满意、可重复和有效的细胞治疗方法。
