BHF Centre for Cardiovascular Science, Queens Medical Research Institute, University of Edinburgh, Edinburgh, UK.
School of Pharmacy, Biodiscovery Institute, University of Nottingham, University Park, Nottingham, UK.
Diabet Med. 2023 Dec;40(12):e15192. doi: 10.1111/dme.15192. Epub 2023 Aug 17.
Our aim was to determine if ultrasound-guided HPV injection in mice would provide reproducible and reliable results, as is currently obtained via open laparotomy techniques, and offer a surgical refinement to emulate islet transplantation in humans.
Fluorescent-polymer microparticles (20 μm) were injected (27G-needle) into the HPV via open laparotomy (n = 4) or under ultrasound-guidance (n = 4) using an MX550D-transducer with a Vevo3100-scanner (FUJIFILM VisualSonics, Inc.). Mice were culled 24-h post injection; organs were frozen, step sectioned (10 μm-slices) and 10 sections/mouse (50 μm-spacing) were quantified for microparticles in the liver and other organs by fluorescent microscopy.
Murine HPV injection, via open laparotomy-route, resulted in widespread distribution of microparticles in the liver, lungs and spleen; ultrasound-guided injection resulted in reduced microparticle delivery (p < 0.0001) and microparticle clustering in distinct areas of the liver at the site of needle penetration, with very few/no microparticles being seen in lung and spleen tissues, hypothesised to be due to flow into the body cavity: liver median (interquartile range) 4.15 (0.00-4.15) versus 0.00 (0.00-0.00) particle-count mm , respectively.
Ultrasound-guided injection results in microparticle clustering in the liver, with an overall reduction in microparticle number when compared to open laparotomy HPV injection, and high variability in microparticle-counts detected between mice. Ultrasound-guided injection is not currently a technique that can replace open laparotomy HPV of islet transplantation in mice.
我们旨在确定在小鼠中进行超声引导的 HPV 注射是否能够提供与目前通过剖腹手术技术获得的可重复且可靠的结果，并提供一种手术改良方法，以模拟人类胰岛移植。
通过剖腹手术（n=4）或超声引导（n=4）将荧光聚合物微球（20μm）用 27G 针注射到 HPV 中，使用带有 Vevo3100 扫描仪的 MX550D 换能器（FUJIFILM VisualSonics，Inc.）。注射后 24 小时处死小鼠；将器官冷冻，分步切片（10μm 切片），每只小鼠 10 个切片（50μm 间隔），通过荧光显微镜对肝脏和其他器官中的微球进行定量。
通过剖腹手术途径进行的 HPV 注射导致微球在肝脏、肺和脾脏中广泛分布；超声引导的注射导致微球传递减少（p<0.0001），并且微球在针穿刺部位的肝脏特定区域聚集，在肺和脾脏组织中几乎看不到/没有微球，这被假设是由于微球流入体腔：肝脏中位数（四分位间距）4.15（0.00-4.15）与 0.00（0.00-0.00）的颗粒计数/mm ，分别。
超声引导的注射导致肝脏中微球聚集，与剖腹手术 HPV 注射相比，微球数量总体减少，并且在不同小鼠之间检测到的微球计数存在很大差异。目前，超声引导的注射技术不能替代剖腹手术 HPV 对小鼠胰岛移植。
