血清脂联素和皮质醇水平不受所研究的 ADIPOQ 基因变异的影响：德黑兰血脂和血糖研究。

Serum adiponectin and cortisol levels are not affected by studied ADIPOQ gene variants: Tehran lipid and glucose study.

机构信息

Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran.

Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

出版信息

BMC Endocr Disord. 2022 Apr 18;22(1):104. doi: 10.1186/s12902-022-01020-8.

DOI:10.1186/s12902-022-01020-8

PMID:35436947

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9016932/

Abstract

BACKGROUND

Obesity is a major public health concern in developed and even developing countries worldwide. Adiponectin is a protein secreted by adipose tissue that modulates many metabolic processes and plays a vital role in obesity. This study aimed to determine the association of four variants of the ADIPOQ gene with serum adiponectin, cortisol levels and obesity status.

METHODS

This case-control study was performed on 164 obese individuals compared by 156 control from the Tehran Lipid and Glucose Study (TLGS). Standard procedures obtained anthropometric measures and metabolic parameters. Cortisol and adiponectin levels were measured by ELISA method. rs1501299, rs266729, rs17300539, and rs17366743 on the ADIPOQ gene were genotyped using the PCR-RFLP. The correlation between adiponectin gene SNPs and obesity were calculated by Additive, dominant, and recessive genetic models. Pearson's or Spearman's found correlations between adiponectin levels and metabolic and anthropometric variables. Data were analyzed using SPSS software Version 20.

RESULTS

Adiponectin and cortisol levels were significantly lower in obese subjects compared to the control group (p < 0.05). There was a significant negative correlation between serum adiponectin level and BMI, waist circumference (WC), waist-hip ratio, hip circumference (HC), Fasting blood sugar (FBS) Triglyceride (TG), Total cholesterol (TC), Systolic blood pressure (SBP), Diastolic blood pressure (DBP) (r = - 0.147, r = - 0.324, r = 0.371, r = - 0.179, r = - 0.299, r = - 0.277, r = - 0.041, r = - 0.134, and r = - 0.149, respectively). A positive correlation was found between adiponectin and high-density lipoprotein cholesterol (HDL-C) (r = 0.29), but no significant correlations were found between adiponectin and Low-density lipoprotein cholesterol(LDL-C) and cortisol. ADIPOQ variant rs1501299 was significantly associated with cortisol levels in subjects with BMI ≥ 25 (P-value =0.039).

CONCLUSIONS

Adiponectin and cortisol levels were associated with obesity. No ADIPOQ gene variants and haplotypes were associated with cortisol, Adiponectin, and obesity.

摘要

背景

肥胖是全世界发达国家甚至发展中国家的一个主要公共卫生问题。脂联素是一种由脂肪组织分泌的蛋白质，可调节多种代谢过程，在肥胖中起着至关重要的作用。本研究旨在确定 ADIPOQ 基因的四个变体与血清脂联素、皮质醇水平和肥胖状况的关联。

方法

本病例对照研究比较了来自德黑兰血脂和血糖研究（TLGS）的 164 名肥胖个体和 156 名对照者。标准程序获得了人体测量学和代谢参数。采用 ELISA 法测量皮质醇和脂联素水平。使用 PCR-RFLP 技术对 ADIPOQ 基因上的 rs1501299、rs266729、rs17300539 和 rs17366743 进行基因分型。通过加性、显性和隐性遗传模型计算脂联素基因 SNP 与肥胖的相关性。用 Pearson 或 Spearman 分析了脂联素水平与代谢和人体测量变量之间的相关性。使用 SPSS 软件版本 20 分析数据。

结果

与对照组相比，肥胖组的脂联素和皮质醇水平显著降低（p<0.05）。血清脂联素水平与 BMI、腰围（WC）、腰臀比、臀围（HC）、空腹血糖（FBS）、甘油三酯（TG）、总胆固醇（TC）、收缩压（SBP）、舒张压（DBP）呈显著负相关（r=-0.147，r=-0.324，r=-0.371，r=-0.179，r=-0.299，r=-0.277，r=-0.041，r=-0.134，r=-0.149）。脂联素与高密度脂蛋白胆固醇（HDL-C）呈正相关（r=0.29），但脂联素与低密度脂蛋白胆固醇（LDL-C）和皮质醇之间无显著相关性。ADIPOQ 变体 rs1501299 与 BMI≥25 的受试者的皮质醇水平显著相关（P 值=0.039）。