血清素在经细胞内分析和染料注射的海龟视网膜神经节细胞中的免疫细胞化学定位。

Immunocytochemical localization of serotonin in intracellularly analyzed and dye-injected ganglion cells of the turtle retina.

作者信息

Weiler R, Ammermüller J

出版信息

Neurosci Lett. 1986 Dec 12;72(2):147-52. doi: 10.1016/0304-3940(86)90070-4.

Abstract

Combining intracellular recording techniques with immunocytochemistry revealed the existence of serotonin within a few ganglion cells of the turtle retina. Lucifer yellow was intracellularly injected into physiologically classified ganglion cells. Sections containing labeled somata were subsequently incubated with an antibody directed against serotonin and immunoreactivity was revealed using horseradish peroxidase as a marker. In 3 ganglion cells the two markers were co-localized. These cells ramified in layers 2 and 3 of the inner plexiform layer and produced color opponent ON-OFF photoresponses.

摘要

将细胞内记录技术与免疫细胞化学相结合，揭示了乌龟视网膜少数神经节细胞中存在5-羟色胺。将荧光黄细胞内注射到生理分类的神经节细胞中。随后，含有标记细胞体的切片与抗5-羟色胺抗体一起孵育，并使用辣根过氧化物酶作为标记物显示免疫反应性。在3个神经节细胞中，这两种标记物共定位。这些细胞在内网状层的第2层和第3层分支，并产生颜色拮抗的开-关光反应。