Department of Radiology, Faculty of ParaMedicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Technology of Radiology and Radiotherapy, Allied Medical Sciences School, Tehran University of Medical Sciences, Tehran, Iran.
Curr Radiopharm. 2023;16(1):71-77. doi: 10.2174/1874471015666220418133919.
Some compounds have been investigated to mitigate the effect of radiation on the lung, such as pneumonitis and fibrosis.
This study aimed to examine the mitigation efficiency of Spirulina compared to the effect of Metformin.
25 male Wistar rats were allotted in five groups: control, Spirulina, Radiation, Radiation plus Spirulina, and Radiation plus Metformin. Rat chest regions were irradiated by 15 Gray (Gy) xradiation using aLINAC. Forty-eight hours after irradiation, treatment with Spirulina and Metformin began. Eighty days after irradiation, all rats were sacrificed, and their lung tissues were removed for histopathological, and biochemical assays.
The results demonstrated that irradiation increased MDA (Malondialdehyde) levels while suppressing the SOD (superoxide dismutase) and GPx(glutathione peroxidase) activity in the irradiated group. MDA levels in lung tissues were reduced with Metformin but not with Spirulina. Both Metformin and Spirulina increased the SOD and GPx activity in lung tissue. Moreover, histopathological evaluations showed extensive changes in the lung tissue including infiltration of lymph cells around the bronchioles and blood vessels, thickening of the alveolar wall, and the disruption of the alveolar structure, as well as accumulation of collagen fibers. Administration of Spirulina and Metformin significantly reduced pathological changes in lung tissue, although the effect of Metformin was greater than that of Spirulina.
Spirulina could mitigate radiation-induced lung injury moderately, although Metformin is more effective than Spirulina as a mitigator agent.
一些化合物已被研究用于减轻辐射对肺的影响，如放射性肺炎和肺纤维化。
本研究旨在检验螺旋藻与二甲双胍相比的缓解效果。
将 25 只雄性 Wistar 大鼠分为五组：对照组、螺旋藻组、放射组、放射加螺旋藻组和放射加二甲双胍组。使用 LINAC 对大鼠胸部区域进行 15 戈瑞（Gy）X 射线照射。照射后 48 小时，开始用螺旋藻和二甲双胍进行治疗。照射后 80 天，所有大鼠被处死，取出肺组织进行组织病理学和生化分析。
结果表明，照射组 MDA（丙二醛）水平升高，而 SOD（超氧化物歧化酶）和 GPx（谷胱甘肽过氧化物酶）活性降低。MDA 水平在肺组织中用二甲双胍降低，但用螺旋藻没有降低。二甲双胍和螺旋藻均能增加肺组织中的 SOD 和 GPx 活性。此外，组织病理学评估显示肺组织广泛变化，包括细支气管和血管周围淋巴细胞浸润、肺泡壁增厚、肺泡结构破坏以及胶原纤维积累。螺旋藻和二甲双胍的给药显著减轻了肺组织的病理变化，尽管二甲双胍的效果大于螺旋藻。
螺旋藻可以适度减轻辐射引起的肺损伤，尽管二甲双胍作为缓解剂比螺旋藻更有效。
