Shah Furqan A, Jolic Martina, Micheletti Chiara, Omar Omar, Norlindh Birgitta, Emanuelsson Lena, Engqvist Håkan, Engstrand Thomas, Palmquist Anders, Thomsen Peter
Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Department of Materials Science and Engineering, McMaster University, Hamilton, Ontario, Canada.
Bioact Mater. 2022 Apr 6;19:103-114. doi: 10.1016/j.bioactmat.2022.03.012. eCollection 2023 Jan.
Calcium phosphates (CaP) represent an important class of osteoconductive and osteoinductive biomaterials. As proof-of-concept, we show how a multi-component CaP formulation (monetite, beta-tricalcium phosphate, and calcium pyrophosphate) guides osteogenesis beyond the physiological envelope. In a sheep model, hollow dome-shaped constructs were placed directly over the occipital bone. At 12 months, large amounts of bone (∼75%) occupy the hollow space with strong evidence of ongoing remodelling. Features of both compact bone (osteonal/osteon-like arrangements) and spongy bone (trabeculae separated by marrow cavities) reveal insights into function/need-driven microstructural adaptation. Pores within the CaP also contain both woven bone and vascularised lamellar bone. Osteoclasts actively contribute to CaP degradation/removal. Of the constituent phases, only calcium pyrophosphate persists within osseous (cutting cones) and non-osseous (macrophages) sites. From a translational perspective, this multi-component CaP opens up exciting new avenues for osteotomy-free and minimally-invasive repair of large bone defects and augmentation of the dental alveolar ridge.
磷酸钙（CaP）是一类重要的具有骨传导性和骨诱导性的生物材料。作为概念验证，我们展示了一种多组分CaP配方（一水磷酸氢钙、β-磷酸三钙和焦磷酸钙）如何在生理范围之外引导骨生成。在绵羊模型中，将空心圆顶形构建体直接放置在枕骨上方。12个月时，大量骨组织（约75%）占据了空心空间，有明显的持续重塑迹象。致密骨（骨单位/类骨单位排列）和松质骨（由骨髓腔分隔的小梁）的特征揭示了功能/需求驱动的微观结构适应性。CaP内的孔隙还包含编织骨和血管化板层骨。破骨细胞积极参与CaP的降解/清除。在组成相中，只有焦磷酸钙在骨内（切割锥）和非骨内（巨噬细胞）部位持续存在。从转化医学的角度来看，这种多组分CaP为大骨缺损的无截骨和微创修复以及牙槽嵴增高开辟了令人兴奋的新途径。
