Gallinetti Sara, Linder Lars Kihlström Burenstam, Åberg Jonas, Illies Christopher, Engqvist Håkan, Birgersson Ulrik
Department of Engineering Sciences, Applied Materials Science Section, Uppsala University, 75121 Uppsala, Sweden.
OssDsign AB, 75450 Uppsala, Sweden.
Biomed Mater. 2021 Mar 5;16(3). doi: 10.1088/1748-605X/abca12.
In a 52 week ovine calvaria implantation model, the restoration of cranial defects with a bare titanium mesh (Ti-mesh) and a titanium mesh embedded in a calcium phosphate (CaP-Ti) were evaluated in seven animals. During the study, no major clinical abnormalities were observed, and all sheep presented a normal neurologic assessment. Blood and cerebrospinal fluid analysis, made at termination, did not show any abnormalities. No indentation of the soft tissue was observed for either test article; however, the Ti-mesh burr-hole covers were associated with filling of the calvarial defect by fibrous tissue mainly. Some bone formation was observed at the bottom of the created defect, but no significant bone was formed in the proximity of the implant. The defect sites implanted with CaP-Ti were characterized by a moderate degradation of the calcium phosphate (CaP) that was replaced by mature bone tissue. Calcium-phosphate-filled macrophages were observed in all animals, indicating that they might play a vital role in osteogenesis. The newly formed bone was present, especially at the bony edges of the defect and on the dura side. Integration of the Ti-mesh in a CaP improved bone formation and osteointegration in comparison to a bare Ti-mesh.
在一个为期52周的绵羊颅骨植入模型中，对7只动物使用裸钛网（Ti网）和嵌入磷酸钙的钛网（CaP-Ti）修复颅骨缺损的情况进行了评估。在研究过程中，未观察到重大临床异常，所有绵羊的神经学评估均正常。在实验结束时进行的血液和脑脊液分析未显示任何异常。两种测试物品均未观察到软组织凹陷；然而，Ti网钻孔覆盖物主要与纤维组织填充颅骨缺损有关。在创建的缺损底部观察到一些骨形成，但在植入物附近未形成明显的骨。植入CaP-Ti的缺损部位的特征是磷酸钙（CaP）适度降解，被成熟骨组织取代。在所有动物中均观察到充满磷酸钙的巨噬细胞，表明它们可能在骨生成中起重要作用。新形成的骨存在，尤其是在缺损的骨边缘和硬脑膜一侧。与裸Ti网相比，将Ti网整合到CaP中可改善骨形成和骨整合。
