Institute of Plant Biology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
Agricultural Experiment Station of Zhejiang University, Hangzhou, Zhejiang, China.
Plant Signal Behav. 2022 Dec 31;17(1):2065432. doi: 10.1080/15592324.2022.2065432.
The paralogous genes () are important for root and panicle development in rice ( L.). However, the specific role of paralogous genes is still not clear. To understand the specific roles of paralogs in rice, we generated triple and quadruple mutants by crossing the and double mutants which we previously created. Compared with the 7-day-old wild type, the and triple mutants showed no obvious phenotype variation except that the triple mutant had shorter primary root and shoot. The and triple mutants exhibited a series of developmental abnormalities, including shorter primary roots, longer root hairs, fewer crown roots and lateral roots, shorter and curved shoots. Furthermore, the quadruple mutant displayed more severe phenotypic defects which was lethal. In addition, the expression levels of some hormone signal transduction and crown root development related genes, such as , and , were significantly altered in the stem base of all examined multiple mutants. Taken together, our results demonstrated that the four paralogous genes function redundantly in regulating rice growth and development.
在水稻（L.）中，拟基因对根和穗发育很重要。然而，拟基因的具体作用仍不清楚。为了了解拟基因在水稻中的具体作用，我们通过杂交先前创建的和双突变体来生成三重和四重突变体。与 7 天龄的野生型相比，三重突变体除了主根和茎较短外，没有明显的表型变化。三重和四重突变体表现出一系列发育异常，包括主根变短、根毛变长、冠根和侧根减少、茎变短变弯。此外，四倍体突变体表现出更严重的表型缺陷，是致死的。此外，在所有检查的多重突变体的茎基部，一些激素信号转导和冠根发育相关基因，如、和，的表达水平显著改变。总之，我们的结果表明，四个拟基因在调控水稻生长发育中具有冗余功能。
