Veress B, el Hassan A M, Kutty M K, Al-Gindan Y, Kubba R, Omer A E
Trop Geogr Med. 1986 Dec;38(4):380-5.
The distribution and numbers of IgG-, IgA-, IgM-, and lysozyme-positive cells were investigated by the immunoperoxidase method in paraffin-sections of 13 cases of chronic cutaneous leishmaniasis of low parasite load from Saudi Arabia. The majority of the peroxidase-positive plasma cells contained IgG, whereas the numbers of IgA+ and IgM+ plasma cells were not so numerous. Small groups of squamous epithelial cells showed immunoreactivity for IgG and IgA. Similar positive staining was observed extracellularly in the oedematous upper dermis, in the endothelial cells, and in the perivascular space. The activated macrophages showed strong and diffuse peroxidase staining for lysozyme, whereas epithelioid cells and multinucleated giant cells were negative or had finely granular and considerably weaker staining. It is suggested that humoral immunity also participates in the elimination of the parasites and an immunologically induced necrosis might be responsible for the ulceration of the skin in cutaneous leishmaniasis. It is also assumed that the lysozyme immunoreactivity can be a marker of the activation state of the macrophages.
采用免疫过氧化物酶法，对来自沙特阿拉伯的13例低寄生虫负荷的慢性皮肤利什曼病石蜡切片中IgG、IgA、IgM和溶菌酶阳性细胞的分布及数量进行了研究。大多数过氧化物酶阳性浆细胞含有IgG，而IgA+和IgM+浆细胞数量较少。小群鳞状上皮细胞对IgG和IgA呈免疫反应性。在水肿的真皮上层、内皮细胞和血管周围间隙的细胞外也观察到类似的阳性染色。活化的巨噬细胞对溶菌酶呈强而弥漫的过氧化物酶染色，而上皮样细胞和多核巨细胞为阴性或呈细颗粒状且染色明显较弱。提示体液免疫也参与了寄生虫的清除，免疫诱导的坏死可能是皮肤利什曼病皮肤溃疡的原因。还推测溶菌酶免疫反应性可作为巨噬细胞活化状态的标志物。
