Martinotti M G, Arione R, Foà R, Pegoraro L, Jemma C, Forni G
Tumori. 1986 Dec 31;72(6):553-8. doi: 10.1177/030089168607200603.
A human acute T lymphoblastic leukemia line (PF-382) was serially transplanted into nude mice. No takes were observed in untreated nude mice, whereas solid tumors were observed in splenectomized and total body, sublethally irradiated mice. The minimal tumor-inducing dose and the latency time remained unchanged after the third and fifth serial transplants. Moreover, leukemic cells recovered from the 8th in vivo passages displayed the same differentiation antigens and chromosomal markers as the in vitro PF-382 cell line used for the first transplant. This stable and well-characterized experimental system could be a new model for T-lymphocyte differentiation and immune-reactivity against human leukemias.
将一株人类急性T淋巴细胞白血病细胞系（PF - 382）连续移植到裸鼠体内。在未经处理的裸鼠中未观察到肿瘤生长，而在脾切除并接受全身亚致死剂量照射的小鼠中观察到实体瘤。在第三次和第五次连续移植后，最小肿瘤诱导剂量和潜伏期保持不变。此外，从第8次体内传代回收的白血病细胞与首次移植所用的体外PF - 382细胞系表现出相同的分化抗原和染色体标记。这个稳定且特征明确的实验系统可能成为T淋巴细胞分化以及针对人类白血病的免疫反应性研究的新模型。
