Zhang Junfei, Du Yuanxin, Zhang Yongcai, Xu Yanan, Fan Yanying, Li Yan
Shanxi Provincial People's Hospital Affiliated to Shanxi Medical University, Taiyuan, 030001, People's Republic of China.
Department of Pharmacology, Basic Medical Sciences Center, Shanxi Medical University, Taiyuan, 030001, People's Republic of China.
Cancer Manag Res. 2022 Apr 14;14:1457-1469. doi: 10.2147/CMAR.S348981. eCollection 2022.
Lung metastasis is a common metastasis site of colorectal cancer which largely reduces the quality of life and survival rates of patients. The discovery of potential novel diagnostic biomarkers is very meaningful for the early diagnosis of colorectal cancer with lung metastasis.
In the present study, the metabonomic profiling of serum samples of lung metastasis mice was analyzed by H-nuclear magnetic resonance (H-NMR). Principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to elucidate the distinguishing metabolites between different groups, and all achieved excellent separations, which indicated that metastatic mice could be differentiated from control mice based on the metabolic profiles at serum levels. Furthermore, during lung metastasis of colorectal cancer, metabolic phenotypes changed significantly, and some of metabolites were identified.
Among these metabolites, approximately 15 were closely associated with the lung metastasis process. Pathway enrichment analysis results showed deregulation of metabolic pathways participating in the process of lung metastasis, such as synthesis and degradation of ketone bodies pathway, amino acid metabolism pathway and pyruvate metabolism pathway.
The present study demonstrated the metabolic disturbances of serum samples of mice during the lung metastasis process of colorectal cancer and provides potential diagnostic biomarkers for the disease.
肺转移是结直肠癌常见的转移部位，这在很大程度上降低了患者的生活质量和生存率。发现潜在的新型诊断生物标志物对于结直肠癌肺转移的早期诊断具有重要意义。
在本研究中，通过氢核磁共振（H-NMR）分析肺转移小鼠血清样本的代谢组学特征。采用主成分分析（PCA）、偏最小二乘判别分析（PLS-DA）和正交偏最小二乘判别分析（OPLS-DA）来阐明不同组之间的差异代谢物，所有分析均实现了良好的分离效果，这表明基于血清水平的代谢谱可以区分转移小鼠和对照小鼠。此外，在结直肠癌肺转移过程中，代谢表型发生了显著变化，并且鉴定出了一些代谢物。
在这些代谢物中，约有15种与肺转移过程密切相关。通路富集分析结果显示参与肺转移过程的代谢通路失调，如酮体合成与降解通路、氨基酸代谢通路和丙酮酸代谢通路。
本研究证明了结直肠癌肺转移过程中小鼠血清样本的代谢紊乱，并为该疾病提供了潜在的诊断生物标志物。
