Wei Bo, Zeng Meiyu, Yang Jing, Li Shuainan, Zhang Jiantao, Ding Nan, Jiang Zhisheng
Research Lab of Translational Medicine, Hengyang Medical School, University of South China, Hengyang, China.
Key Laboratory for Arteriosclerology of Hunan Province, Human International Scientific and Technological Cooperation Base of Arteriosclerotic Disease, Institute of Cardiovascular Disease, Hengyang Medical College, University of South China, Hengyang, China.
Front Cell Dev Biol. 2022 Apr 4;10:835205. doi: 10.3389/fcell.2022.835205. eCollection 2022.
Stem cell transplantation (SCT) holds great promise for overcoming diseases by regenerating damaged cells, tissues and organs. The potential for self-renewal and differentiation is the key to SCT. RNA methylation, a dynamic and reversible epigenetic modification, is able to regulate the ability of stem cells to differentiate and regenerate. -methyladenosine (mA) is the richest form of RNA methylation in eukaryotes and is regulated by three classes of proteins: methyltransferase complexes, demethylase complexes and mA binding proteins. Through the coordination of these proteins, RNA methylation precisely modulates the expression of important target genes by affecting mRNA stability, translation, selective splicing, processing and microRNA maturation. In this review, we summarize the most recent findings on the regulation of mA modification in embryonic stem cells, induced pluripotent stem cells and adult stem cells, hoping to provide new insights into improving SCT technology.
干细胞移植（SCT）在通过再生受损细胞、组织和器官来攻克疾病方面具有巨大潜力。自我更新和分化的能力是干细胞移植的关键。RNA甲基化是一种动态且可逆的表观遗传修饰，能够调节干细胞的分化和再生能力。N6-甲基腺苷（m6A）是真核生物中最丰富的RNA甲基化形式，受三类蛋白质调节：甲基转移酶复合物、去甲基酶复合物和m6A结合蛋白。通过这些蛋白质的协同作用，RNA甲基化通过影响mRNA稳定性、翻译、选择性剪接、加工和微小RNA成熟来精确调节重要靶基因的表达。在本综述中，我们总结了胚胎干细胞、诱导多能干细胞和成体干细胞中m6A修饰调控的最新研究发现，希望为改进干细胞移植技术提供新的见解。
