Karabulut Sebahattin, Filiz Ahmet Kemal, Akkaya Recep
Department of Medical Services and Techniques, Vocational School of Health Services, Sivas Cumhuriyet University, Sivas, Turkey.
Department of Medical Physiology, Faculty of Medicine, Sivas Cumhuriyet University, Sivas, Turkey.
Neurol Res. 2022 Oct;44(10):902-909. doi: 10.1080/01616412.2022.2066785. Epub 2022 Apr 21.
Epileptogenesis, the process by which the brain becomes epileptic, is related to neuroinflammation, hyperexcitability cognitive deficits. Evidence suggests that improving brain inflammation can inhibit the epileptogenesis process and help the emergence of new drugs for the treatment of epilepsy. Therefore, the PTZ kindling model of epilepsy was utilized to assess the neuroprotective role of thiamine in epileptogenesis.
Male rats were exposed to PTZ-induced kindling and pretreated with low thiamine (25 mg/kg) or high thiamine (50 mg/kg). Cyclooxygenase (COX-1 and COX-2), interleukin 1-beta (IL-1β), tumor necrosis factor-alpha (TNF-α), and nuclear factor-κB (NF-κB) concentrations in the brain were analyzed using biochemical assays. Cognitive function was evaluated using the passive avoidance test.
Thiamine ameliorated epileptogenesis and enhanced the rats' performance in the passive avoidance test. Also, thiamine significantly decreased the level of neuroinflammatory mediators in the brain induced by PTZ.
These results provide evidence that thiamine alleviates PTZ-induced neuroinflammation and cognitive impairments.
癫痫发生是大脑发生癫痫的过程，与神经炎症、过度兴奋和认知缺陷有关。有证据表明，改善脑部炎症可抑制癫痫发生过程，并有助于开发治疗癫痫的新药。因此，利用戊四氮点燃癫痫模型评估硫胺素在癫痫发生中的神经保护作用。
雄性大鼠接受戊四氮诱导的点燃，并分别用低剂量硫胺素（25毫克/千克）或高剂量硫胺素（50毫克/千克）进行预处理。使用生化分析方法分析大脑中环氧合酶（COX-1和COX-2）、白细胞介素1-β（IL-1β）、肿瘤坏死因子-α（TNF-α）和核因子-κB（NF-κB）的浓度。使用被动回避试验评估认知功能。
硫胺素改善了癫痫发生，并提高了大鼠在被动回避试验中的表现。此外，硫胺素显著降低了戊四氮诱导的大脑中神经炎症介质的水平。
这些结果证明硫胺素可减轻戊四氮诱导的神经炎症和认知障碍。
