Hubei Key Laboratory of Plasma Chemistry and Advanced Materials, School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan 430205, China.
School of Biomedical Engineering, Huazhong University of Science and Technology (HUST), Wuhan 430074, China.
ACS Appl Mater Interfaces. 2022 May 4;14(17):19480-19490. doi: 10.1021/acsami.2c02119. Epub 2022 Apr 21.
The ultrasensitive determination of sulfate reducing bacteria (SRB) is of great significance for their crucial roles in environmental and industrial harms together with the early detection of microbial corrosion. In this work, we report the development of highly efficient electrocatalysts, CuO-CuO extended hexapods (EHPs), which are wrapped on homemade freestanding graphene paper to construct a flexible paper electrode in the electrochemical sensing of the biomarker sulfide for SRB detection. Herein CuO-CuO EHPs have been synthesized a highly controllable and facile approach at room temperature, where the redox centers of copper oxide nanoarchitectures are tuned facet engineering, and then they are deposited on the graphene paper surface through an electrostatic adsorption to enable homogeneous and highly dense distribution. Owing to the synergistic contribution of high electrocatalytic activity from the Cu mixed oxidation states and abundant catalytically active facets of CuO-CuO EHPs and high electrical conductivity of the graphene paper electrode substrate, the resultant nanohybrid paper electrode has exhibited superb electrochemical sensing properties for HS with a wide linear range up to 352 μM and an extremely low detection limit (LOD) of 0.1 nM with a signal-to-noise ratio of 3 (/ = 3), as well as high sensitivity, stability, and selectivity. Furthermore, taking advantage of the good biocompatibility and mechanical flexibility, the electrochemical sensing platform based on the proposed electrode has been applied in the sensitive detection of SRB in environmental samples through the sensing of sulfide from SRB, which holds great promise for on-site and online corrosion and environmental monitoring.
硫酸盐还原菌（SRB）的超灵敏检测对于其在环境和工业危害中的关键作用以及微生物腐蚀的早期检测具有重要意义。在这项工作中，我们报告了高效电催化剂 CuO-CuO 扩展六足体（EHPs）的开发，它包裹在自制的独立式石墨烯纸上，以构建用于 SRB 检测的生物标志物硫化物电化学传感的柔性纸电极。在此，通过室温下的高度可控和简便方法合成了 CuO-CuO EHPs，其中氧化铜纳米结构的氧化还原中心通过面工程进行了调谐，然后通过静电吸附沉积在石墨烯纸表面上，以实现均匀和高密度的分布。由于 Cu 混合氧化态和 CuO-CuO EHPs 的丰富催化活性面的高电催化活性以及石墨烯纸电极基底的高导电性的协同贡献，所得纳米杂化纸电极对 HS 表现出极好的电化学传感性能，线性范围高达 352 μM，检测限（LOD）低至 0.1 nM（信噪比为 3），具有高灵敏度、稳定性和选择性。此外，利用良好的生物相容性和机械柔韧性，基于所提出的电极的电化学传感平台已通过从 SRB 中检测硫化物，用于环境样品中 SRB 的灵敏检测，这为现场和在线腐蚀和环境监测提供了很大的前景。
