Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, P. R. China.
Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
ACS Appl Mater Interfaces. 2021 Feb 10;13(5):6023-6033. doi: 10.1021/acsami.0c20645. Epub 2021 Jan 26.
The specific monitoring of serotonin (ST) has provoked massive interest in therapeutic and biological science since it has been recognized as the third most significant endogenous gastrointestinal neurotransmitter. Hence, there is a great need to develop a sensitive and low-cost sensing platform for the detection of a clinically relevant ST level in biological matrices. Herein, we develop a simple two-step approach for an ultrasensitive electrochemical (EC) sensor with the CuO metal oxide (MO)-incorporated CNT core that has been further deposited with a transitional amount of platinum nanoparticles (Pt NPs). We presented, for the first time, the deposition of Pt NPs on the (CNTs-CuO-CuO) nanopetal composite the galvanic replacement method, where copper not only acts as a reductant but a sacrificial template as well. The electrocatalytic aptitude of the fabricated EC sensing platform has been assessed for the sensitive detection of ST as a proficient biomarker in early disease diagnostics. The synergy of improved active surface area, remarkable conductivity, polarization effect induced by Pt NPs on CNTs-CuO-CuO nanopetals, fast electron transfer, and mixed-valence states of copper boost up the redox processes at the electrode-analyte junction. The CNTs-CuO-CuO@Pt-modified electrode has unveiled outstanding electrocatalytic capabilities toward ST oxidation in terms of a low detection limit of 3 nM (S/N = 3), wide linear concentration range, reproducibility, and incredible durability. Owing to the amazing proficiency, the proposed EC sensor based on the CNTs-CuO-CuO@Pt heterostructure has been applied for ST detection in biotic fluids and real-time tracking of ST efflux released from various cell lines as early disease diagnostic approaches.
由于血清素 (ST) 被认为是第三大重要的内源性胃肠道神经递质，因此对其进行特异性监测在治疗学和生物学领域引起了极大的兴趣。因此，非常需要开发一种用于检测生物基质中临床相关 ST 水平的灵敏且低成本的传感平台。在此，我们开发了一种简单的两步法，用于构建具有 CNT 核的氧化铜 (MO) 掺入的 CuO 金属氧化物 (MO) 的超灵敏电化学 (EC) 传感器，进一步沉积了适量的铂纳米粒子 (Pt NPs)。我们首次提出了在 (CNTs-CuO-CuO) 纳米花瓣复合材料上沉积 Pt NPs 的方法，即通过置换反应将铜沉积在 CNTs-CuO-CuO 纳米花瓣上，其中铜不仅作为还原剂，而且还作为牺牲模板。所制备的 EC 传感平台的电催化性能已被评估用于作为早期疾病诊断中灵敏生物标志物的 ST 敏感检测。Pt NPs 在 CNTs-CuO-CuO 纳米花瓣上诱导的增加的有效表面积、显著的导电性、极化效应、快速电子转移和铜的混合价态，增强了电极-分析物界面处的氧化还原过程。与 CNTs-CuO-CuO 相比， CNTs-CuO-CuO@Pt 修饰电极对 ST 氧化具有出色的电催化能力，其检测限低至 3 nM（S/N = 3），线性浓度范围宽，重现性好，耐用性强。由于其出色的性能，基于 CNTs-CuO-CuO@Pt 异质结构的 EC 传感器已被用于生物流体中的 ST 检测，并实时跟踪来自各种细胞系的 ST 流出物，作为早期疾病诊断方法。
