Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
Department of Pediatrics, Graduate School of Medicine, Chiba University, Chiba, Chiba 260-8677, Japan.
J Proteome Res. 2022 May 6;21(5):1340-1348. doi: 10.1021/acs.jproteome.2c00099. Epub 2022 Apr 21.
Previously, we performed nontargeted proteome analysis using dried blood spots (DBSs) that are widely used in newborn screening for the clinical diagnosis of congenital genetic diseases and immunodeficiency. We have developed an efficient and simple pretreatment method for DBSs that can detect more than 1000 proteins. To complement proteins that are difficult to detect via DBS analysis with less invasive alternative body fluids, we conducted this study to investigate the proteins detected from dried saliva spots (DSSs) using single-shot LC-MS/MS, which is practical in clinical settings. We also clarified whether DSSs have the same advantages as DBSs, and we investigated the influence of saliva collection conditions and the storage environment on their protein profile. As a result, we detected approximately 5000 proteins in DSSs and whole saliva, and we concluded that they were sufficient to complement the proteins lacking in the blood analysis. DSSs could be used as an alternative tool to DBSs for detecting the presence of causative proteins.
此前，我们使用广泛应用于新生儿筛查以临床诊断先天性遗传疾病和免疫缺陷的干血斑（DBS）进行非靶向蛋白质组分析。我们已经开发出一种高效简单的 DBS 预处理方法，可检测 1000 多种蛋白质。为了用更微创的替代体液补充 DBS 分析中难以检测到的蛋白质，我们进行了这项研究，使用单次 LC-MS/MS 检测从干唾液斑（DSS）中检测到的蛋白质，这种方法在临床环境中实用。我们还阐明了 DSS 是否具有与 DBS 相同的优势，并研究了唾液采集条件和储存环境对其蛋白质谱的影响。结果，我们在 DSS 和全唾液中检测到约 5000 种蛋白质，并得出结论，它们足以补充血液分析中缺失的蛋白质。DSS 可以作为 DBS 的替代工具，用于检测致病蛋白的存在。
