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古蛋白组学鉴定挪威乌达尔小教堂壁画用颜料的原始黏合剂和现代污染物。

Palaeoproteomic identification of the original binder and modern contaminants in distemper paints from Uvdal stave church, Norway.

机构信息

SFF Centre for Early Sapiens Behaviour (SapienCE), University of Bergen, Bergen, Norway.

Globe Institute, University of Copenhagen, Copenhagen, Denmark.

出版信息

Sci Rep. 2024 Jun 4;14(1):12858. doi: 10.1038/s41598-024-63455-4.

DOI:10.1038/s41598-024-63455-4
PMID:38834702
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11150381/
Abstract

Two distemper paint samples taken from decorative boards in Uvdal stave church, Norway, were analysed using palaeoproteomics, with an aim of identifying their binder and possible contaminants. The results point at the use of calfskin to produce hide glue as the original paint binder, and are consistent with the instructions of binder production and resource allocation in the historical records of Norway. Although we did not observe any evidence of prior restoration treatments using protein-based materials, we found abundant traces of human saliva proteins, as well as a few oats and barley peptides, likely deposited together on the boards during their discovery in the 1970s. This work illustrates the need to fully consider contamination sources in palaeoproteomics and to inform those working with such objects about the potential for their contamination.

摘要

从挪威乌达尔 stave 教堂的装饰板上取了两个动物疫病涂料样本,使用古蛋白组学进行了分析,目的是确定其粘合剂和可能的污染物。结果表明使用牛皮来生产动物胶作为原始涂料粘合剂,这与挪威历史记录中粘合剂生产和资源分配的说明一致。尽管我们没有观察到任何使用基于蛋白质的材料进行先前修复处理的证据,但我们发现了丰富的人唾液蛋白痕迹,以及一些燕麦和大麦肽,这些可能是在 20 世纪 70 年代发现这些木板时一起沉积在上面的。这项工作说明了在古蛋白组学中充分考虑污染来源的必要性,并告知那些处理此类物品的人注意其可能受到污染的情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd3/11150381/e1d305693157/41598_2024_63455_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd3/11150381/78e25c6dcca7/41598_2024_63455_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd3/11150381/08fda2fed377/41598_2024_63455_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd3/11150381/e1d305693157/41598_2024_63455_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd3/11150381/78e25c6dcca7/41598_2024_63455_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd3/11150381/08fda2fed377/41598_2024_63455_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1fd3/11150381/e1d305693157/41598_2024_63455_Fig3_HTML.jpg

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