Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Hangzhou 310058, China.
Department of Physiology, Anatomy and Microbiology, La Trobe University, Melbourne Campus, Bundoora, VIC 3086, Australia; Centre for Future Landscapes, La Trobe University, Melbourne Campus, Bundoora, VIC 3086, Australia.
Sci Total Environ. 2022 Aug 15;834:155261. doi: 10.1016/j.scitotenv.2022.155261. Epub 2022 Apr 18.
Atrazine has a detrimental effect on soybean growth in corn-soybean rotation systems. A knowledge gap exists regarding how rhizosphere microbial interactions respond to atrazine stress, and specifically, whether they may alleviate the detriment of atrazine on soybeans, this serving as a target to alleviate the adverse impact. Biochar are widely used for remediation in herbicide contamination soil, however, little is known about how biochar fuels the microbiomes in rhizosphere to improve soybean performance. We investigated the response of the microbial community to atrazine stress with and without biochar application to soybean cultivation in a greenhouse experiment. Atrazine had detrimental effects on soybeans and nodules, reshaping the microbial community in both the bulk and rhizosphere soil. Biochar application was able to ameliorate atrazine effects on soybean and nodule activity, with an increase in competition among microbes in the soybean rhizosphere soils. Biochar favored the probiotics such as the bacteria Lysobacter, Paenarthrobacter, and Sediminibacterium in the rhizosphere soils. The relative abundance of Lysobacter exhibited strong-negative correlations with potential pathogens. Elastic net regression with bioindicators and environmental factors accurately predicted the residual content of atrazine in soil. Collectively, our results provide a practical strategy of using biochar to improve soil quality for corn-soybean rotation that is contaminated with residual atrazine. Overall, beneficial plant microbes and changes in microbial interactions and assembly processes in the soybean rhizosphere are capable of alleviating atrazine stress on soybean growth.
莠去津对玉米-大豆轮作系统中大豆的生长有不利影响。目前尚不清楚根际微生物相互作用如何应对莠去津胁迫，特别是它们是否可以减轻莠去津对大豆的危害，这是缓解其不利影响的目标。生物炭广泛用于受除草剂污染土壤的修复，但对于生物炭如何激发根际微生物群落来改善大豆性能知之甚少。我们在温室实验中研究了在大豆种植过程中添加和不添加生物炭对微生物群落对莠去津胁迫的响应。莠去津对大豆和根瘤有不利影响，重塑了土壤和根际土壤中的微生物群落。生物炭的应用能够减轻莠去津对大豆和根瘤活力的影响，同时增加了大豆根际土壤中微生物之间的竞争。生物炭有利于根际土壤中的有益菌，如细菌 Lysobacter、Paenarthrobacter 和 Sediminibacterium。Lysobacter 的相对丰度与潜在病原体呈强烈负相关。使用生物标志物和环境因素进行弹性网络回归可以准确预测土壤中莠去津的残留含量。总的来说，我们的研究结果为利用生物炭改善受莠去津残留污染的玉米-大豆轮作土壤质量提供了一种实用策略。总体而言，有益的植物微生物以及根际微生物相互作用和组装过程的变化能够缓解莠去津对大豆生长的胁迫。
