Neffe Lisa, Abendroth Lisa, Bautsch Willfried, Häussler Susanne, Tomasch Jürgen
Department of Molecular Bacteriology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Städtisches Klinikum Braunschweig gGmbH, Braunschweig, Germany.
Genomics. 2022 May;114(3):110368. doi: 10.1016/j.ygeno.2022.110368. Epub 2022 Apr 18.
Plasmid-encoded antibiotic resistance encompasses many classes of currently used antibiotics. In globally distributed Escherichia coli lineages plasmids, which spread via horizontal gene transfer, are responsible for the dissemination of genes encoding extended-spectrum β-lactamases (ESBL). In this study, we combined 2nd and 3rd generation sequencing techniques to reconstruct the plasmidome of overall 97 clinical ESBL-E. coli isolates. Our results highlight the enormous plasmid diversity in respect to size, replicon-type and genetic content. Furthermore, we emphasize the diverse plasmid distribution patterns among the clinical isolates and the high intra- and extracellular mobility potential of resistance conferring genes. While the majority of resistance conferring genes were located on large plasmids of known replicon type, small cryptic plasmids seem to be underestimated resistance gene vectors. Our results contribute to a better understanding of the dissemination of resistance-conferring genes through horizontal gene transfer as well as clonal spread.
质粒编码的抗生素耐药性涵盖了许多目前使用的抗生素类别。在全球分布的大肠杆菌谱系中，通过水平基因转移传播的质粒是编码超广谱β-内酰胺酶（ESBL）基因传播的原因。在本研究中，我们结合第二代和第三代测序技术，重建了总共97株临床产ESBL大肠杆菌分离株的质粒组。我们的结果突出了质粒在大小、复制子类型和遗传内容方面的巨大多样性。此外，我们强调了临床分离株之间不同的质粒分布模式以及耐药基因在细胞内和细胞外的高移动潜力。虽然大多数耐药基因位于已知复制子类型的大质粒上，但小型隐蔽质粒似乎是被低估的耐药基因载体。我们的结果有助于更好地理解耐药基因通过水平基因转移以及克隆传播的情况。
