Messele Yohannes E, Trott Darren J, Hasoon Mauida F, Veltman Tania, McMeniman Joe P, Kidd Stephen P, Djordjevic Steven P, Petrovski Kiro R, Low Wai Y
The Davies Livestock Research Centre, The University of Adelaide, Adelaide, SA 5371, Australia.
The Australian Centre for Antimicrobial Resistance Ecology, The University of Adelaide, Adelaide, SA 5005, Australia.
Antibiotics (Basel). 2023 May 11;12(5):895. doi: 10.3390/antibiotics12050895.
The similarity of commensal isolated from healthy cattle to antimicrobial-resistant bacteria causing extraintestinal infections in humans is not fully understood. In this study, we used a bioinformatics approach based on whole genome sequencing data to determine the genetic characteristics and phylogenetic relationships among faecal isolates from beef cattle (n = 37) from a single feedlot in comparison to previously analysed pig faecal (n = 45), poultry extraintestinal (n = 19), and human extraintestinal isolates (n = 40) from three previous Australian studies. Most beef cattle and pig isolates belonged to phylogroups A and B1, whereas most avian and human isolates belonged to B2 and D, although a single human extraintestinal isolate belonged to phylogenetic group A and sequence type (ST) 10. The most common sequence types (STs) included ST10 for beef cattle, ST361 for pig, ST117 for poultry, and ST73 for human isolates. Extended-spectrum and AmpC β-lactamase genes were identified in seven out of thirty-seven (18.9%) beef cattle isolates. The most common plasmid replicons identified were IncFIB (AP001918), followed by IncFII, Col156, and IncX1. The results confirm that feedlot cattle isolates examined in this study represent a reduced risk to human and environmental health with regard to being a source of antimicrobial-resistant of clinical importance.
从健康牛分离出的共生菌与导致人类肠道外感染的耐药菌之间的相似性尚未完全明确。在本研究中，我们基于全基因组测序数据采用生物信息学方法，以确定来自单个饲养场的肉牛粪便分离株（n = 37）与之前三项澳大利亚研究中分析过的猪粪便分离株（n = 45）、家禽肠道外分离株（n = 19）和人类肠道外分离株（n = 40）之间的遗传特征和系统发育关系。大多数肉牛和猪的分离株属于A群和B1群，而大多数禽类和人类分离株属于B2群和D群，不过有一株人类肠道外分离株属于系统发育A群和序列型（ST）10。最常见的序列型包括肉牛的ST10、猪的ST361、家禽的ST117和人类分离株的ST73。在37株肉牛分离株中有七株（18.9%）鉴定出了超广谱和AmpC β-内酰胺酶基因。鉴定出的最常见质粒复制子是IncFIB（AP001918），其次是IncFII、Col156和IncX1。结果证实，本研究中检测的饲养场牛分离株作为具有临床重要性的耐药菌来源，对人类和环境健康构成的风险较低。
