Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Int J Food Microbiol. 2022 Jul 2;372:109678. doi: 10.1016/j.ijfoodmicro.2022.109678. Epub 2022 Apr 12.
Multi-mycotoxin contamination of barley reduces malt quality and also poses serious health risks to both humans and animals. Among the detected mycotoxins in barley, Fusarium and Alternaria toxins represent severe food safety issues due to their widespread prevalence and strong synergistic toxicity. In this study, the effects of Fusarium and Alternaria fungi and their related toxins were investigated using simulated malting process conditions for contaminated barley samples. The dynamic changes of the fungal community and their associated mycotoxins were analyzed via high-throughput sequencing and UHPLC-q-trap-MS/MS, respectively. The results showed significant differences in the fungal communities between barley grains and their associated malt samples. These differences were noted for the following: fungal community diversity, dominant flora and the fungal structures at phylum, genus, and family level. Additionally, significant changes in the levels of alternariol monomethyl ether, tentoxin, zearalenone, and 15Acetyl-Deoxynivelenone were observed (p < 0.05) during the malting process. These results provide valuable information for strategies aimed at controlling fungal infections and the presence of mycotoxins in malt, so as to ensure food safety and human health.
多菌灵污染的大麦降低了麦芽的质量，也对人类和动物的健康构成了严重威胁。在大麦中检测到的真菌毒素中，镰刀菌和交链孢霉毒素由于其广泛存在和强烈的协同毒性，是严重的食品安全问题。在这项研究中，使用受污染的大麦样品的模拟制麦过程条件研究了镰刀菌和交链孢霉真菌及其相关毒素的影响。通过高通量测序和 UHPLC-q-trap-MS/MS 分别分析了真菌群落及其相关真菌毒素的动态变化。结果表明，大麦籽粒与其相关麦芽样品之间的真菌群落存在显著差异。这些差异表现在真菌群落多样性、优势菌群以及门、属和科水平的真菌结构上。此外，在制麦过程中还观察到 alternariol monomethyl ether、tentoxin、zearalenone 和 15Acetyl-Deoxynivelenone 的水平发生了显著变化（p < 0.05）。这些结果为控制麦芽中真菌感染和真菌毒素存在的策略提供了有价值的信息，以确保食品安全和人类健康。
